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Literature summary for 1.8.4.8 extracted from

  • Kopriva, S.; Buchert, T.; Fritz, G.; Suter, M.; Benda, R.; Schunemann, V.; Koprivova, A.; Schurmann, P.; Trautwein, A.X.; Kroneck, P.M.H.; Brunold, C.
    The presence of an iron-sulfur cluster in adenosine 5'-phosphosulfate reductase separates organisms utilizing adenosine 5'-phosphosulfate and phosphoadenosine 5'-phosphosulfate for sulfate assimilation (2002), J. Biol. Chem., 277, 21786-21791.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, phylogenetic analysis, overexpression in strain BL21(DE3) as His-tagged protein Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
additional information enzyme contains no iron Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylyl-sulfate + thioredoxin Escherichia coli
-
adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite
-
?
additional information Escherichia coli enzyme is essential for sulfate assimilation using phosphoadenosine 5'-phosphosulfate, not adenosine 5'-phosphosulfate, phylogenetic comparison of the 2 different groups, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl-sulfate + thioredoxin
-
Escherichia coli adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite
-
?
3'-phosphoadenylyl-sulfate + thioredoxin thioredoxin m from spinach Escherichia coli adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite
-
?
additional information enzyme is essential for sulfate assimilation using phosphoadenosine 5'-phosphosulfate, not adenosine 5'-phosphosulfate, phylogenetic comparison of the 2 different groups, overview Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
3'-Phosphoadenylylsulfate reductase
-
Escherichia coli
PAPS reductase
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
thioredoxin
-
Escherichia coli