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Literature summary for 1.8.4.B3 extracted from

  • Ding, D.; Sagher, D.; Laugier, E.; Rey, P.; Weissbach, H.; Zhang, X.H.
    Studies on the reducing systems for plant and animal thioredoxin-independent methionine sulfoxide reductases B (2007), Biochem. Biophys. Res. Commun., 361, 629-633.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Nicotiana tabacum

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Nicotiana tabacum 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15200
-
x * 15200, calculated from sequence Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptide-L-methionine-(R)-S-sulfoxide + thionein Nicotiana tabacum
-
peptide-L-methionine + thionein disulfide + H2O
-
?

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum A7U630 precursor, fragment
-

Purification (Commentary)

Purification (Comment) Organism
-
Nicotiana tabacum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Nicotiana tabacum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dabsyl-L-methionine (R)-S-sulfoxide + bovine liver thionein NtMsrB1 Nicotiana tabacum dabsyl-L-methionine + bovine liver thionein + H2O
-
?
dabsyl-L-methionine (R)-S-sulfoxide + dithiothreitol NtMsrB1 Nicotiana tabacum dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
?
dabsyl-L-methionine (R)-S-sulfoxide + plant Trx-like protein CDSP32 NtMsrB1 Nicotiana tabacum dabsyl-L-methionine + ?
-
?
dabsyl-L-methionine (R)-S-sulfoxide + selenocysteamine NtMsrB1 Nicotiana tabacum dabsyl-L-methionine + 2,2'-disulfanediyldiethanamine + H2O
-
?
additional information NtMsrB1 shows very little activity towards L-methionine (R)-sulfoxide. Escherichia coli thioredoxin can not serve as a reducing agent for NtMsrB1. The Trx-independent MsrB enzyme lacks an additional cysteine (resolving cysteine) that is capable of forming a disulfide bond on the enzyme during the catalytic reaction Nicotiana tabacum ?
-
?
peptide-L-methionine-(R)-S-sulfoxide + thionein
-
Nicotiana tabacum peptide-L-methionine + thionein disulfide + H2O
-
?

Subunits

Subunits Comment Organism
? x * 15200, calculated from sequence Nicotiana tabacum

Synonyms

Synonyms Comment Organism
NtMsrB1
-
Nicotiana tabacum
thioredoxin-independent methionine sulfoxide reductases B
-
Nicotiana tabacum