Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, 34% loss of activity | Pleurotus ostreatus | |
Cd2+ | 5 mM CdCl2, complete loss of activity | Pleurotus ostreatus | |
Cu2+ | 0.1 mM CuSO4, 43% loss of activity | Pleurotus ostreatus | |
Fe2+ | 0.1 mM FeSO4, 34% loss of activity | Pleurotus ostreatus | |
HgCl2 | 5 mM HgCl2, 94% loss of activity | Pleurotus ostreatus | |
imidazole | 1 mM, 14% loss of activity | Pleurotus ostreatus | |
iodoacetic acid | 1 mM, 26% loss of activity | Pleurotus ostreatus | |
Mn2+ | 5 mM MnSO4, 82% loss of activity | Pleurotus ostreatus | |
NEM | 1 mM, 23% loss of activity | Pleurotus ostreatus | |
PCMB | 1 mM, 32% loss of activity | Pleurotus ostreatus | |
Sodium azide | 1 mM, 32% loss of activity | Pleurotus ostreatus | |
Zn2+ | 5 mM ZnSO4, 50% loss of activity | Pleurotus ostreatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
GSH | - |
Pleurotus ostreatus | |
2.5 | - |
dehydroascorbate | - |
Pleurotus ostreatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Pleurotus ostreatus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
86000 | - |
gel filtration | Pleurotus ostreatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus ostreatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pleurotus ostreatus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Pleurotus ostreatus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.3 | - |
- |
Pleurotus ostreatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GSH + dehydroascorbate | specific for glutathione as hydrogen donor | Pleurotus ostreatus | GSSG + ascorbate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 86000, SDS-PAGE | Pleurotus ostreatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | 45 | - |
Pleurotus ostreatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 6.2, 1 h, stable below | Pleurotus ostreatus |
65 | - |
complete loss of activity above | Pleurotus ostreatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pleurotus ostreatus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | pH 6.5: about 25% of maximal activity, pH 8.5: about 60% of maximal activity | Pleurotus ostreatus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
highest stability at, unstable under acidic and highly alkaline conditions | Pleurotus ostreatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | enzyme shows an unusual flavin peak, enzyme might form a flavin adduct | Pleurotus ostreatus |