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Literature summary for 1.8.5.1 extracted from
- Mechanism of the reaction catalyzed by dehydroascorbate reductase from spinach chloroplasts (2003), Eur. J. Biochem., 270, 921-928.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C23S | mutant enzyme has almost no activity | Spinacia oleracea |
| C26S | turnover number is 57% of the wild-type value, KM-value for dehydroascorbate is 2fold lower than the wild-type value, KM-value for GSH is 1.6old lower than the wild-type value | Spinacia oleracea |
| C9S | turnover number is 86% of the wild-type value, KM-value for dehydroascorbate is 2.8fold lower than the wild-type value, KM-value for GSH is 2fold lower than the wild-type value | Spinacia oleracea |
| C9S/C26S | turnover number is 43% of the wild-type value, KM-value for dehydroascorbate is 1.1fold higher than the wild-type value, KM-value for GSH is identical to the wild-type value | Spinacia oleracea |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.019 | - |
dehydroascorbate | mutant enzyme C9S | Spinacia oleracea |  |
| 0.026 | - |
dehydroascorbate | mutant enzyme C23S | Spinacia oleracea | |
| 0.053 | - |
dehydroascorbate | wild-type enzyme | Spinacia oleracea | |
| 0.058 | - |
dehydroascorbate | - |
Spinacia oleracea | |
| 0.69 | - |
GSH | mutant enzyme C23S | Spinacia oleracea | |
| 0.95 | - |
GSH | mutant enzyme C9S | Spinacia oleracea | |
| 1.1 | - |
GSH | wild-type enzyme | Spinacia oleracea | |
| 1.1 | - |
GSH | mutant enzyme C9S/C26S | Spinacia oleracea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Spinacia oleracea | 9507 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GSH + dehydroascorbate | - |
Spinacia oleracea | GSSG + ascorbate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DHAR | - |
Spinacia oleracea |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 210 | - |
dehydroascorbate | - |
Spinacia oleracea | |
| 210 | - |
GSH | mutant enzyme C9S/C26S | Spinacia oleracea | |
| 280 | - |
GSH | mutant enzyme C23S | Spinacia oleracea | |
| 280 | - |
dehydroascorbate | mutant enzyme C23S | Spinacia oleracea | |
| 420 | - |
GSH | mutant enzyme C9S | Spinacia oleracea | |
| 420 | - |
dehydroascorbate | mutant enzyme C9S | Spinacia oleracea | |
| 490 | - |
GSH | wild-type enzyme | Spinacia oleracea | |
| 490 | - |
dehydroascorbate | wild-type enzyme | Spinacia oleracea | |
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Release 2023.1 (January 2023)
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