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Literature summary for 1.8.98.1 extracted from

  • Heiden, S.; Hedderich, R.; Setzke, E.; Thauer, R.K.
    Purification of a cytochrome b containing H2:heterodisulfide oxidoreductase complex from membranes of Methanosarcina barkeri (1993), Eur. J. Biochem., 213, 529-535.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound to Methanosarcina barkeri 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the H2:heterodisulfide oxidoreductase multienzyme complex contains 70-80 nmol non-heme iron and acid labile sulfur per mg of protein Methanosarcina barkeri
Ni the H2:heterodisulfide oxidoreductase multienzyme complex contains 0.6 nmol Ni per mg of protein Methanosarcina barkeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
MW of H2:heterodisulfide oxidoreductase multienzyme complex is 800000-1300000, gel filtration Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 Methanosarcina barkeri energy-conserving step in methanohenic archaea coenzyme B + coenzyme M
-
?

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanosarcina barkeri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6
-
reduction of the heterodisulfide with H2 Methanosarcina barkeri
24
-
reduction of the heterodisulfide with benzylviologen Methanosarcina barkeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2
-
Methanosarcina barkeri coenzyme B + coenzyme M
-
?
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 energy-conserving step in methanohenic archaea Methanosarcina barkeri coenzyme B + coenzyme M
-
?
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced benzyl viologen
-
Methanosarcina barkeri coenzyme B + coenzyme M + benzyl viologen
-
?

Subunits

Subunits Comment Organism
? the H2:heterodisulfide oxidoreductase multienzyme complex is composed of nine polypepetides with MW 46000 Da, 39000 Da, 28000 Da, 25000 Da, 23000 Da, 21000 Da, 20000 Da, 16000 Da, 15000 Da, SDS-PAGE Methanosarcina barkeri

Synonyms

Synonyms Comment Organism
H2:heterodisulfide oxidoreductase
-
Methanosarcina barkeri
More the H2:heterodisulfide oxidoreductase multienzyme complex is composed of nine polypeptides with MW 46000 Da, 39000 Da, 28000 Da, 25000 Da, 23000 Da, 21000 Da, 20000 Da, 16000 Da, 15000 Da Methanosarcina barkeri

Cofactor

Cofactor Comment Organism Structure
cytochrome b the H2:heterodisulfide oxidoreductase multienzyme complex contains 3.2 nmol cytochrome b per mg protein Methanosarcina barkeri
FAD the H2:heterodisulfide oxidoreductase multienzyme complex contiams 0.6 nmol per mg of protein Methanosarcina barkeri
additional information the H2:heterodisukfide oxidoreductase complex does not mediate the reduction of coenzyme F420 with H2 nor the oxidation of reduced coenzyme F420 with the heterodisulfide Methanosarcina barkeri