KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-value for N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine is below 0.1 mM | Methanothermobacter thermautotrophicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Methanothermobacter thermautotrophicus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Methanothermobacter thermautotrophicus Marburg / DSM 2133 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanothermobacter thermautotrophicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.2 | - |
- |
Methanothermobacter thermautotrophicus |
Storage Stability | Organism |
---|---|
4°C, under N2, partially purified enzyme, 50 mM Tris-HCl, pH 7.6, 0.5 M NaCl, loss of 20% activity within 24 h | Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 | - |
Methanothermobacter thermautotrophicus | coenzyme B + coenzyme M | - |
? | |
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 | - |
Methanothermobacter thermautotrophicus Marburg / DSM 2133 | coenzyme B + coenzyme M | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
rapid inactivation above | Methanothermobacter thermautotrophicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | - |
Pipes/KOH buffer | Methanothermobacter thermautotrophicus |
7.6 | - |
20°C, Tris-HCl buffer | Methanothermobacter thermautotrophicus |