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Literature summary for 1.8.99.2 extracted from
- Substrate phosphorylation in Chlorobium vibrioforme f. sp. thiosulfatophilum (1983), J. Gen. Microbiol., 129, 1365-1370.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | above 2 mM | Prosthecochloris vibrioformis |  |
| iodoacetamide | 5 mM, 54% inhibition | Prosthecochloris vibrioformis | |
| NEM | 5 mM, 49% inhibition | Prosthecochloris vibrioformis | |
| PCMB | 1 mM, 30% inhibition | Prosthecochloris vibrioformis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
AMP | - |
Prosthecochloris vibrioformis | |
| 0.17 | - |
sulfite | - |
Prosthecochloris vibrioformis | |
| 0.25 | - |
adenylyl sulfate | - |
Prosthecochloris vibrioformis | |
| 4.2 | - |
ferricyanide | - |
Prosthecochloris vibrioformis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | contains 4-6 mol of non-haem iron per mol of enzyme | Prosthecochloris vibrioformis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 180000 | - |
gel filtration | Prosthecochloris vibrioformis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Prosthecochloris vibrioformis | - |
f. sp. thiosulfatophilum | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Prosthecochloris vibrioformis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.15 | - |
- |
Prosthecochloris vibrioformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| AMP + sulfite + ferricyanide | - |
Prosthecochloris vibrioformis | adenylyl sulfate + ferrocyanide | - |
? | |
| GMP + sulfite + ferricyanide | 54% of the activity with AMP | Prosthecochloris vibrioformis | guanylyl sulfate + ferrocyanide | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Prosthecochloris vibrioformis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Prosthecochloris vibrioformis | |
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