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Literature summary for 1.8.99.2 extracted from
- Adenylylsulfate reductase of Chlorobium limicola (1974), Arch. Microbiol., 100, 115-120.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| 30-50% loss of activity by repeated freezing and thawing | Chlorobium limicola |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.091 | - |
ferricyanide | - |
Chlorobium limicola |  |
| 0.2 | - |
AMP | - |
Chlorobium limicola | |
| 0.91 | - |
sulfite | - |
Chlorobium limicola | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | contains nonheme iron | Chlorobium limicola | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 210000 | - |
gel filtration | Chlorobium limicola |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlorobium limicola | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Chlorobium limicola |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.75 | - |
- |
Chlorobium limicola |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for several weeks | Chlorobium limicola |
| 4°C, more than 2 days, more than 30% loss of activity | Chlorobium limicola |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| AMP + sulfite + ferricyanide | - |
Chlorobium limicola | adenylyl sulfate + ferrocyanide | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
2 h, more than 30% loss of activity | Chlorobium limicola |
| 60 | - |
heating for longer periods results in protein precipitation with simultaneous loss of activity | Chlorobium limicola |
| 70 | - |
2 min, 50% loss of activity | Chlorobium limicola |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.7 | - |
- |
Chlorobium limicola |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | contains 1.4 mol of FAD per mol of enzyme | Chlorobium limicola | |
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Release 2023.1 (January 2023)
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