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Literature summary for 1.8.99.2 extracted from
- Adenylylsulfate reductases from thiobacilli (1994), Methods Enzymol., 243, 393-400.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NEM | 10 mM, complete inhibition | Thiobacillus denitrificans |  |
| NEM | 5 mM complete inhibition | Thiobacillus thioparus | |
| PCMB | 0.1 mM, 30% inhibition | Thiobacillus denitrificans | |
| PCMB | 1 mM, complete inhibition | Thiobacillus thioparus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0025 | - |
AMP | reaction with cytochrome c | Thiobacillus thioparus | |
| 0.01 | - |
GMP | reaction with cytochrome c | Thiobacillus thioparus | |
| 0.017 | - |
sulfite | reaction with cytochrome c | Thiobacillus thioparus | |
| 0.041 | - |
AMP | reaction with ferricyanide | Thiobacillus denitrificans | |
| 0.1 | - |
AMP | reaction with ferricyanide | Thiobacillus thioparus | |
| 0.63 | - |
GMP | reaction with ferricyanide | Thiobacillus denitrificans | |
| 1.5 | - |
sulfite | reaction with ferricyanide | Thiobacillus denitrificans | |
| 2.5 | - |
sulfite | reaction with ferricyanide | Thiobacillus thioparus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | contains 8-10 mol of non-heme iron per mol of enzyme | Thiobacillus thioparus | |
| Iron | contains 8-13 mol of non-heme iron per mol of enzyme | Thiobacillus denitrificans | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thiobacillus denitrificans | - |
- |
- |
| Thiobacillus thioparus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thiobacillus thioparus |
- |
Thiobacillus denitrificans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.4 | - |
- |
Thiobacillus thioparus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 5°C, 5 days, 20% loss of activity | Thiobacillus thioparus |
| frozen, protein concentration above 5 mg/ml, stable for several months | Thiobacillus denitrificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| AMP + sulfite + cytochrome c | - |
Thiobacillus thioparus | adenylyl sulfate + ? | - |
? | |
| AMP + sulfite + ferricyanide | - |
Thiobacillus thioparus | adenylyl sulfate + ferrocyanide | - |
? | |
| AMP + sulfite + ferricyanide | - |
Thiobacillus denitrificans | adenylyl sulfate + ferrocyanide | - |
? | |
| CMP + sulfite + ferricyanide | - |
Thiobacillus thioparus | cytidylyl sulfate + ferrocyanide | - |
? | |
| CMP + sulfite + ferricyanide | no activity with CMP | Thiobacillus denitrificans | cytidylyl sulfate + ferrocyanide | - |
? | |
| deoxyAMP + sulfite + ferricyanide | - |
Thiobacillus thioparus | deoxyadenylyl sulfate + ferrocyanide | - |
? | |
| GMP + sulfite + cytochrome c | - |
Thiobacillus thioparus | guanylyl sulfate + ? | - |
? | |
| GMP + sulfite + ferricyanide | - |
Thiobacillus denitrificans | guanylyl sulfate + ferrocyanide | - |
? | |
| UMP + sulfite + ferricyanide | - |
Thiobacillus thioparus | uridylyl sulfate + ferrocyanide | - |
? | |
| UMP + sulfite + ferricyanide | no activity with UMP | Thiobacillus denitrificans | uridylyl sulfate + ferrocyanide | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Thiobacillus denitrificans |
| 60 | - |
6 min, stable | Thiobacillus thioparus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
ferricyanide assay, phosphate buffer | Thiobacillus thioparus |
| 7.2 | - |
- |
Thiobacillus denitrificans |
| 7.4 | - |
ferricyanide assay, Tris-HCl buffer | Thiobacillus thioparus |
| 9.5 | - |
reaction with cytochrome c as electron acceptor, Tris-HCl buffer | Thiobacillus thioparus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | noncovalently bound | Thiobacillus thioparus | |
| FAD | noncovalently bound | Thiobacillus denitrificans | |
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