Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
C139S | no detectable activity | Pseudomonas aeruginosa |
C140S | 3% activity of the wild type enzyme | Pseudomonas aeruginosa |
C228S | no detectable activity | Pseudomonas aeruginosa |
C231S | no detectable activity | Pseudomonas aeruginosa |
C256S | no detectable activity | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
thioredoxin | thioredoxin originating from Clamydomonas reinhardtii | Pseudomonas aeruginosa | |
8.6 | - |
thioredoxin | thioredoxin originating from Escherichia coli | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30470 | - |
MALDI-TOF mass spectrometry, the enzyme used is a recombinant protein with 48 additional residues containing the His6 tag | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.302 | - |
mutant enzyme C140S, at 30°C in 100 mM Tris-HCl buffer pH 8.0 containing 1 mM EDTA, 5 mM DTT, 0.106 mM thioredoxin from Escherichia coli, and 0.05 mM APS | Pseudomonas aeruginosa |
10.3 | - |
wild type enzyme, at 30°C in 100 mM Tris-HCl buffer pH 8.0 containing 1 mM EDTA, 5 mM DTT, 0.106 mM thioredoxin from Escherichia coli, and 0.05 mM APS | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenylylsulfate + thioredoxin | - |
Pseudomonas aeruginosa | AMP + sulfite + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
5'-adenylylsulfate reductase | - |
Pseudomonas aeruginosa |
APR | - |
Pseudomonas aeruginosa |