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Literature summary for 1.8.99.B1 extracted from
- Functional properties of the protein disulfide oxidoreductase from the archaeon Pyrococcus furiosus: a member of a novel protein family related to protein disulfide-isomerase (2004), Eur. J. Biochem., 271, 3437-3448.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant enzymes C35S, C146S and C35S/C146S in Escherichia coli | Pyrococcus furiosus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C146S | not active in the insulin reductase assay, oxidation of the dithiol peptide NRCSQGSCWN is reduced | Pyrococcus furiosus |
| C35S | active in the insulin reductase assay, oxidation of the dithiol peptide NRCSQGSCWN is comparable to wild-type enzyme | Pyrococcus furiosus |
| C35S/C146S | not active in the insulin reductase assay, no oxidation of the dithiol peptide NRCSQGSCWN | Pyrococcus furiosus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25650 | - |
wild-type enzyme electrospray mass spectroscopy | Pyrococcus furiosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protein-dithiol + oxidized acceptor | Pyrococcus furiosus | intracellular proteins of certain hyperthermophilic archaea are rich in disulfide bonds. Disulfide bonds stabilize many thermostable proteins | protein-disulfide + reduced acceptor | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | Q51760 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dithiol peptide NRCSQGSCWN + acceptor | - |
Pyrococcus furiosus | disulfide peptide NRCSQGSCWN + reduced acceptor | - |
? | |
| protein-dithiol + oxidized acceptor | intracellular proteins of certain hyperthermophilic archaea are rich in disulfide bonds. Disulfide bonds stabilize many thermostable proteins | Pyrococcus furiosus | protein-disulfide + reduced acceptor | - |
? | |
| [insulin]-disulfide + reduced dithiothreitol | - |
Pyrococcus furiosus | [insulin]-dithiol + oxidized dithiothreitol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PfPDO | - |
Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Pyrococcus furiosus |
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Release 2023.1 (January 2023)
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