Cloned (Comment) | Organism |
---|---|
mutant enzymes C34S, C148S and C34S/C148S, are overexpressed in Escherichia coli | Aquifex aeolicus |
Crystallization (Comment) | Organism |
---|---|
crystal structure at 2.4 A resolution | Aquifex aeolicus |
Protein Variants | Comment | Organism |
---|---|---|
C148S | mutant enzyme exhibits a lower activity compared to wild-type enzyme | Aquifex aeolicus |
C34S | mutant enzyme retains nearly wild-type activity | Aquifex aeolicus |
C34S/C148S | mutant enzyme shows no activity | Aquifex aeolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26700 | - |
gel-filtration, electrospray mass spectroscopy | Aquifex aeolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | O66753 | - |
- |
Purification (Comment) | Organism |
---|---|
wild-type enzyme and recombinant mutant enzymes C34S, C148S and C34S/C148S | Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme reveals an inherent glutathione-dependent thioltransferase activity | Aquifex aeolicus | ? | - |
? | |
[insulin]-disulfide + reduced dithiothreitol | - |
Aquifex aeolicus | [insulin]-dithiol + oxidized dithiothreitol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AaPDO | - |
Aquifex aeolicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Aquifex aeolicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Aquifex aeolicus |