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Literature summary for 1.8.99.B1 extracted from

  • Pedone, E.; D'Ambrosio, K.; De Simone, G.; Rossi, M.; Pedone, C.; Bartolucci, S.
    Insights on a new PDI-like family: structural and functional analysis of a protein disulfide oxidoreductase from the bacterium Aquifex aeolicus (2005), J. Mol. Biol., 356, 155-164.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzymes C34S, C148S and C34S/C148S, are overexpressed in Escherichia coli Aquifex aeolicus

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure at 2.4 A resolution Aquifex aeolicus

Protein Variants

Protein Variants Comment Organism
C148S mutant enzyme exhibits a lower activity compared to wild-type enzyme Aquifex aeolicus
C34S mutant enzyme retains nearly wild-type activity Aquifex aeolicus
C34S/C148S mutant enzyme shows no activity Aquifex aeolicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26700
-
gel-filtration, electrospray mass spectroscopy Aquifex aeolicus

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus O66753
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type enzyme and recombinant mutant enzymes C34S, C148S and C34S/C148S Aquifex aeolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme reveals an inherent glutathione-dependent thioltransferase activity Aquifex aeolicus ?
-
?
[insulin]-disulfide + reduced dithiothreitol
-
Aquifex aeolicus [insulin]-dithiol + oxidized dithiothreitol
-
?

Synonyms

Synonyms Comment Organism
AaPDO
-
Aquifex aeolicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Aquifex aeolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Aquifex aeolicus