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Literature summary for 1.97.1.1 extracted from
- Study of chlorate-resistant mutants in Escherichia coli K 12 (1971), Biochim. Biophys. Acta, 237, 579-590.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibited by azide | Escherichia coli | |
| p-chloromercuribenzoate | - |
Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
reduced benzyl viologen | - |
Escherichia coli | |
| 0.19 | - |
FMN | - |
Escherichia coli | |
| 0.25 | - |
Chlorate | - |
Escherichia coli | |
| 0.62 | - |
riboflavin | - |
Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| particle-bound | - |
Escherichia coli | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K12, chlorate resistant mutant chl. C- | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, 40% glycerol, 8 d | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chlorate + reduced benzyl viologen | - |
Escherichia coli | chlorite + H2O + benzyl viologen | - |
r | |
| chlorate + reduced electron acceptor | e.g. benzyl viologen, most effective | Escherichia coli | chlorite + H2O + oxidized electron acceptor | - |
? | |
| chlorate + reduced electron acceptor | FMN and riboflavin less effective, not: reduced pyridine nucleotides | Escherichia coli | chlorite + H2O + oxidized electron acceptor | - |
? | |
| chlorite + H2O + FMN | - |
Escherichia coli | chlorate + FMNH2 | - |
r | |
| chlorite + H2O + riboflavin | - |
Escherichia coli | chlorate + reduced rboflavin | - |
r | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
10 min, stable | Escherichia coli |
| 63 | - |
half-life: 12 min | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6.5 | - |
Escherichia coli |
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