Cloned (Comment) | Organism |
---|---|
gene pflA, ligands CcpA, GlnR, and GntR interact with the putative promoters of pflA and pflB, CcpA and GlnR bind to promoters containing cre sites | Streptococcus pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | A0A0H2ZPG2 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
PFL-AE | - |
Streptococcus pneumoniae |
PflA | - |
Streptococcus pneumoniae |
pyruvate formate lyase activating enzyme | - |
Streptococcus pneumoniae |
Organism | Comment | Expression |
---|---|---|
Streptococcus pneumoniae | analysis of the transcriptional regulation of gene pflA, overview | additional information |
General Information | Comment | Organism |
---|---|---|
malfunction | mutation of either pflB, which codes for PFL, or pflA, which codes for pyruvate formate lyase activating enzyme, results in abrogation of mixed acid fermentation on galactose, and leads to a decrease in pneumococcal virulence | Streptococcus pneumoniae |
metabolism | multiple regulators control the transcription of pflA and pflB, some of these regulators are induced by galactose, their control over pflA and pflB is influenced by sodium formate, they exert regulatory influence on each other, and are required for pneumococcal colonization and virulence. Transcriptional profile of mutant DELTApflB compared to the wild-type D39 strain, expression of seven genes annotated as transcriptional regulators is either significantly up or downregulated in the mutant, overview. CcpA, GlnR, and GntR interact with the putative promoters of pflA and pflB, impact of formate on binding affinity of CcpA and GlnR | Streptococcus pneumoniae |
physiological function | pyruvate formate lyase (PFL), converting pyruvate to formate and acetyl-CoA, is the key enzyme for mixed acid fermentation. Active pyruvate formate lyase (PFL) synthesis is finely tuned, and feedback inhibition and activation involving the pyruvate formate lyase activating enzyme, are detected, analysis of the transcriptional regulation of gene pflA, overview. The pyruvate formate lyase activating enzyme (PFL-AE) is responsible for posttranslational activation of inactive PFL | Streptococcus pneumoniae |