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Literature summary for 1.97.1.9 extracted from

  • Schroder, I.; Rech, S.; Krafft, T.; Macy, J.M.
    Purification and characterization of the selenate reductase from Thauera selenatis (1997), J. Biol. Chem., 272, 23765-23768.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
selenate pH 6.0 Thauera selenatis

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Thauera selenatis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron part of at least 2 iron-sulfur centres per enzyme molecule and of the heme group of cytochrome b Thauera selenatis
Iron 12.9 mol per mol of enzyme, based on MW 159000 Thauera selenatis
Molybdenum 1 molybdenum per mol of trimer Thauera selenatis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
1 * 96000, alpha, + 1 * 40000, beta, + 1 * 23000, gamma, SDS-PAGE Thauera selenatis
40000
-
1 * 96000, alpha, + 1 * 40000, beta, + 1 * 23000, gamma, SDS-PAGE Thauera selenatis
96000
-
1 * 96000, alpha, + 1 * 40000, beta, + 1 * 23000, gamma, SDS-PAGE Thauera selenatis
180000
-
gel filtration Thauera selenatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
selenate + electron donor Thauera selenatis selenate is the terminal electron acceptor in dissimilatory selenate reduction selenite + H2O + oxidized electron donor
-
?

Organism

Organism UniProt Comment Textmining
Thauera selenatis
-
selenate-respiring bacterium
-

Purification (Commentary)

Purification (Comment) Organism
57fold, to near homogeneity Thauera selenatis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
41.4
-
purified enzyme Thauera selenatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information NADH, succinate, and lactate are no electron donors Thauera selenatis ?
-
?
additional information no activity with nitrate, nitrite, sulfate, and chlorate Thauera selenatis ?
-
?
selenate + electron donor specific for selenate Thauera selenatis selenite + H2O + oxidized electron donor
-
?
selenate + electron donor selenate is the terminal electron acceptor in dissimilatory selenate reduction Thauera selenatis selenite + H2O + oxidized electron donor
-
?
selenate + reduced benzyl viologen best electron donor Thauera selenatis selenite + H2O + oxidized benzyl viologen
-
?
selenate + reduced methyl viologen 11% of the activity with benzyl viologen Thauera selenatis selenite + H2O + oxidized methyl viologen
-
?

Subunits

Subunits Comment Organism
More 1 cytochrome b per alphabetagamma-complex Thauera selenatis
trimer 1 * 96000, alpha, + 1 * 40000, beta, + 1 * 23000, gamma, SDS-PAGE Thauera selenatis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
387
-
selenate pH 6.0 Thauera selenatis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Thauera selenatis

Cofactor

Cofactor Comment Organism Structure
cytochrome b 1 cytochrome b per alphabetagamma-trimer Thauera selenatis
iron-sulfur centre at least 2 [Fe-S]-centre as prosthetic groups per enzyme molecule Thauera selenatis