Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tungstate | SER isolated from periplasmic fractions from cells grown on 1 mM tungstate display selenate reductase activities with a 20fold reduction in Vmax and a 23fold increase in substrate binding affinity. The thermo-stability and pH dependence of tungsten-SER is shown to be similar to that observed for molybdenum-SER | Thauera selenatis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
selenate | tungsten-SER, pH and temperature not specified in the publication, Vmax: 0.01 micromol/min/mg | Thauera selenatis | |
0.016 | - |
selenate | molybdenum-SER, pH and temperature not specified in the publication, Vmax: 0.2 micromol/min/mg | Thauera selenatis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Molybdenum | - |
Thauera selenatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
1 * 96000 + 1 * 40000 + 1 * 23000, SER consists of three subunits SerA (96 kDa), SerB (40 kDa), and SerC (23 kDa) | Thauera selenatis |
40000 | - |
1 * 96000 + 1 * 40000 + 1 * 23000, SER consists of three subunits SerA (96 kDa), SerB (40 kDa), and SerC (23 kDa) | Thauera selenatis |
96000 | - |
1 * 96000 + 1 * 40000 + 1 * 23000, SER consists of three subunits SerA (96 kDa), SerB (40 kDa), and SerC (23 kDa) | Thauera selenatis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thauera selenatis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
selenate + reduced methyl viologen | - |
Thauera selenatis | selenite + H2O + oxidized methyl viologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 1 * 96000 + 1 * 40000 + 1 * 23000, SER consists of three subunits SerA (96 kDa), SerB (40 kDa), and SerC (23 kDa) | Thauera selenatis |
Synonyms | Comment | Organism |
---|---|---|
selenate reductase | - |
Thauera selenatis |
SER | - |
Thauera selenatis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
optimal temperature for molybdenum-SER | Thauera selenatis |
80 | - |
optimal temperature for tungsten-SER is higher than 80°C | Thauera selenatis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
purified molybdenum-SER complex is stable and active upon heat-shock incubation for 10 min at temperatures up to 60°C. At temperatures greater than 65°C all three subunits (SerABC) are readily denatured | Thauera selenatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Thauera selenatis |