Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli, recombinant protein, intein/chitin-binding fusion constructs, pTBY3 vector | Homo sapiens |
General Stability | Organism |
---|---|
high concentrations of glycerol (40%, v/v) greatly stabilized activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-methionine | stronger inhibitor of BHMT-2 than of BHMT (EC 2.1.1.5) | Homo sapiens | |
additional information | relative inhibition of human BHMT (EC 2.1.1.5) and human BHMT-2 activities by methyl donor substrates determined | Homo sapiens | |
S-adenosyl-L-methionine | weak inhibitor of, does not inhibit BHMT (EC 2.1.1.5), not inhibited by betaine | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.94 | - |
S-methyl-L-methionine | final concentrations of S-methyl-L-methionine ranging from 0.1 mM to 9 mM, lower value than obtained for BHMT (EC 2.1.1.5) | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required for activity | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
SDS-PAGE, 73% sequence identity with the 45 kDa BHMT protein (EC 2.1.1.5) | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration, recombinant protein | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-methyl-L-methionine + L-homocysteine = 2 L-methionine | high homology to betaine-homocysteine methyltransferase (BHMT, EC 2.1.1.5), betaine is not a substrate for BHMT-2 but high activity with S-methyl-L-methionine | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | tissue extracts, activities measured in | Homo sapiens | - |
liver | tissue extracts, activities measured in | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
structural comparison and enzymatic properties of purified human betaine-homocysteine methyltransferase (BHMT, EC 2.1.1.5) and human betaine-homocysteine methyltransferase-2 (BHMT-2) determined, methylation capacities of homocysteine analyzed, S-adenosyl-L-methionine-dependent methylation of homocysteine predicted mostly occurs via BHMT-2 in vivo | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + L-homocysteine | low activity | Homo sapiens | ? | - |
? | |
S-methyl-L-methionine + L-homocysteine | high homology to betaine-homocysteine methyltransferase (BHMT, EC 2.1.1.5), betaine is not a substrate for BHMT-2 but high activity with S-methyl-L-methionine | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BHMT-2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
7.5 | - |
activity assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | similar turnover number like betaine-homocysteine methyltransferase (BHMT, EC 2.1.1.5) | Homo sapiens |