Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta II(DE3) pLysS cells | Haemonchus contortus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
amodiaquine | i.e. 4-[(7-chloroquinolin-4-yl)amino]-2-[(diethylamino)methyl]phenol | Haemonchus contortus | |
diphenhydramine | i.e. 2-(diphenylmethoxy)-N,N-dimethylethanamine | Haemonchus contortus | |
miltefosine | i.e. hexadecyl 2-(trimethylammonio)ethyl phosphate | Haemonchus contortus | |
additional information | isozyme PMT1 is not inhibited by diphenhydramine | Haemonchus contortus | |
sinefungin | - |
Haemonchus contortus | |
tacrine | i.e. 1,2,3,4-tetrahydroacridin-9-amine | Haemonchus contortus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.106 | - |
Phosphoethanolamine | isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | |
0.217 | - |
phosphodimethylethanolamine | isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | |
0.256 | - |
S-adenosyl-L-methionine | isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | |
0.277 | - |
S-adenosyl-L-methionine | isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | |
0.424 | - |
phosphomonomethylethanolamine | isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
1 * 48000, isozyme PMT2, SDS-PAGE | Haemonchus contortus |
49400 | - |
1 * 49400, isozyme PMT2, calculated from amino acid sequence | Haemonchus contortus |
51900 | - |
isozyme PMT2, gel filtration | Haemonchus contortus |
52300 | - |
isozyme PMT1, gel filtration | Haemonchus contortus |
53100 | - |
1 * 53100, isozyme PMT1, calculated from amino acid sequence | Haemonchus contortus |
55000 | - |
1 * 55000, isozyme PMT1, SDS-PAGE | Haemonchus contortus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemonchus contortus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-nitrilotriacetic acid-agarose resin column chromatography and Superdex-200 gel filtration | Haemonchus contortus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 S-adenosyl-L-methionine + phosphoethanolamine | overall reaction | Haemonchus contortus | 3 S-adenosyl-L-homocysteine + phosphocholine | - |
? | |
additional information | isozyme PMT2 does not accept phosphoethanolamine as substrate | Haemonchus contortus | ? | - |
? | |
S-adenosyl-L-methionine + phosphodimethylethanolamine | reaction catalyzed by isozyme PMT2 | Haemonchus contortus | S-adenosyl-L-homocysteine + phosphocholine | - |
? | |
S-adenosyl-L-methionine + phosphoethanolamine | reaction catalyzed by isozyme PMT1 | Haemonchus contortus | S-adenosyl-L-homocysteine + phosphomonomethylethanolamine | - |
? | |
S-adenosyl-L-methionine + phosphomonomethylethanolamine | reaction catalyzed by isozyme PMT2 | Haemonchus contortus | S-adenosyl-L-homocysteine + phosphodimethylethanolamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 48000, isozyme PMT2, SDS-PAGE | Haemonchus contortus |
monomer | 1 * 49400, isozyme PMT2, calculated from amino acid sequence | Haemonchus contortus |
monomer | 1 * 53100, isozyme PMT1, calculated from amino acid sequence | Haemonchus contortus |
monomer | 1 * 55000, isozyme PMT1, SDS-PAGE | Haemonchus contortus |
Synonyms | Comment | Organism |
---|---|---|
phosphoethanolamine methyltransferase | - |
Haemonchus contortus |
PMT1 | isozyme PMT1 contains a methyltransferase domain in the N-terminal half of the protein | Haemonchus contortus |
PMT2 | isozyme PMT2 encodes a C-terminal methyltransferase domain | Haemonchus contortus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.28 | - |
S-adenosyl-L-methionine | isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | |
4.13 | - |
phosphodimethylethanolamine | isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | |
4.9 | - |
phosphomonomethylethanolamine | isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | |
5.82 | - |
S-adenosyl-L-methionine | isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | |
8.63 | - |
Phosphoethanolamine | isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Haemonchus contortus | isozyme PMT1, calculated from amino acid sequence | - |
5.3 |
Haemonchus contortus | isozyme PMT2, calculated from amino acid sequence | - |
5.5 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0125 | - |
isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | miltefosine | |
0.013 | - |
isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | diphenhydramine | |
0.0338 | - |
isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | sinefungin | |
0.0915 | - |
isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | sinefungin | |
0.1252 | - |
isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | miltefosine | |
3.1 | - |
isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | amodiaquine | |
3.4 | - |
isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | amodiaquine | |
3.5 | - |
isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | tacrine | |
23.4 | - |
isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | tacrine |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.24 | - |
S-adenosyl-L-methionine | isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | |
11.56 | - |
phosphomonomethylethanolamine | isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | |
19.05 | - |
phosphodimethylethanolamine | isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | |
22.72 | - |
S-adenosyl-L-methionine | isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | |
81.45 | - |
Phosphoethanolamine | isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus |