Literature summary for 2.1.1.104 extracted from

Hoffmann, L.; Maury, S.; Bergdoll, M.; Thion, L.; Erard, M.; Legrand, M.
Identification of the enzymatic active site of tobacco caffeoyl-coenzyme A O-methyltransferase by site-directed mutagenesis (2001), J. Biol. Chem., 276, 36831-36838.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain TG2	Nicotiana tabacum

Protein Variants

Protein Variants	Comment	Organism
D58A	decrease of catalytic activity	Nicotiana tabacum
Q61S	decrease of catalytic activity	Nicotiana tabacum
R220T	total loss of activity, R220 is involved in the electrostatic interaction with CoA-moiety of the substrate	Nicotiana tabacum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0275	-	caffeoyl-CoA	wild-type	Nicotiana tabacum
0.29	-	caffeoyl-CoA	Q61S mutant	Nicotiana tabacum
0.64	-	caffeoyl-CoA	D58A mutant	Nicotiana tabacum

Organism

Organism	UniProt	Comment	Textmining
Nicotiana tabacum	-	tobacco	-

Purification (Commentary)

Purification (Comment)	Organism
-	Nicotiana tabacum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + caffeoyl-CoA	-	Nicotiana tabacum	S-adenosyl-L-homocysteine + feruloyl-CoA	-	?

Synonyms

Synonyms	Comment	Organism
CCoAOMT	-	Nicotiana tabacum

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Release 2023.1 (January 2023)