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Literature summary for 2.1.1.104 extracted from
- S-Adenosyl-L-methionine:trans-caffeoyl-coenzyme A 3-O-methyltransferase from elicitor-treated parsley cell suspension cultures (1989), Arch. Biochem. Biophys., 271, 488-494.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ethanol | activation, 12% v/v | Petroselinum crispum |  |
| Mg2+ | activation, 0.2 mM | Petroselinum crispum | |
| NaCl | activation, 0.4 M | Petroselinum crispum | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Petroselinum crispum | |
| EDTA | - |
Petroselinum crispum | |
| Mn2+ | - |
Petroselinum crispum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activation, 0.2 mM | Petroselinum crispum | |
| NaCl | activation, 0.4 M | Petroselinum crispum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
gel filtration | Petroselinum crispum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + caffeoyl-CoA | Petroselinum crispum | inducible | S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Petroselinum crispum | - |
parsley | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Petroselinum crispum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | treated with crude cell wall elicitor from Phytophthora megasperma f.sp. glycinea | Petroselinum crispum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.059 | - |
- |
Petroselinum crispum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no substrates are caffeic acid, bergaptol, xanthotoxol, luteolin, esculetin | Petroselinum crispum | ? | - |
? | |
| S-adenosyl-L-methionine + caffeoyl-CoA | trans-caffeoyl-CoA preferred | Petroselinum crispum | S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? | |
| S-adenosyl-L-methionine + caffeoyl-CoA | inducible | Petroselinum crispum | S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? | |
| S-adenosyl-L-methionine + trans-caffeic acid esters | such as methyl caffeate, chlorogenic acid, trans-5-O-caffeoylshikimate, rosmarinic acid, poor substrates | Petroselinum crispum | S-adenosyl-L-homocysteine + ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Petroselinum crispum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Petroselinum crispum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | 8.6 | about half-maximal activity at pH 6.9 and 8.6 | Petroselinum crispum |
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