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Literature summary for 2.1.1.104 extracted from

  • Ma, Q.; Xu, Y.
    Characterization of a caffeic acid 3-O-methyltransferase from wheat and its function in lignin biosynthesis (2008), Biochimie, 90, 515-524.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) Triticum aestivum
recombinant expression in transgenic plants using the transformation by Agrobacterium tumefaciens strain LBA4404 Nicotiana tabacum

Protein Variants

Protein Variants Comment Organism
additional information construction of transgenic COMT knockout Nicotiana tabacum plants by antisense expression of COMT from Triticum aestivum via Agrobacterium tumefaciens transfection, phenotype, overview Triticum aestivum
additional information construction of transgenic COMT knockout Nicotiana tabacum plants by antisense expression of COMT from Triticum aestivum, phenotype, overview Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0173
-
5-hydroxy-coniferaldehyde
-
Triticum aestivum
0.0437
-
caffeoyl aldehyde
-
Triticum aestivum
0.0688
-
caffeic acid
-
Triticum aestivum
0.083
-
caffeoyl-CoA
-
Triticum aestivum
0.0952
-
5-hydroxy-feruloyl-CoA
-
Triticum aestivum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38600
-
x * 38600, about, sequence calculation Triticum aestivum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Triticum aestivum COMT catalyzes the multi-step methylation reactions of hydroxylated monomeric lignin precursors, and plays a pivotal position in the lignin biosynthetic pathway ?
-
?
additional information Nicotiana tabacum COMT catalyzes the multi-step methylation reactions of hydroxylated monomeric lignin precursors, and plays a pivotal position in the lignin biosynthetic pathway ?
-
?
S-adenosyl-L-methionine + caffeoyl-CoA Triticum aestivum
-
S-adenosyl-L-homocysteine + feruloyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum O24149 cv. Wisconsin 38
-
Triticum aestivum Q38J50 cv. H4564
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, expression of antisense enzyme in transgenic Nicotiana tabacum plants Triticum aestivum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
determination of lignin content in transgenic plants, overview Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information COMT catalyzes the multi-step methylation reactions of hydroxylated monomeric lignin precursors, and plays a pivotal position in the lignin biosynthetic pathway Triticum aestivum ?
-
?
additional information COMT catalyzes the multi-step methylation reactions of hydroxylated monomeric lignin precursors, and plays a pivotal position in the lignin biosynthetic pathway Nicotiana tabacum ?
-
?
additional information the enzyme is capable of 3'- and 5'-methylating not only acid, aldehyde and alcohol precursors, but also ester precursors, broad substrate specificity, overview Triticum aestivum ?
-
?
S-adenosyl-L-methionine + 5-hydroxy-feruloyl-CoA
-
Triticum aestivum S-adenosyl-L-homocysteine + sinapoyl-CoA
-
?
S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde
-
Triticum aestivum S-adenosyl-L-homocysteine + sinapaldehyde
-
?
S-adenosyl-L-methionine + caffeic acid
-
Triticum aestivum S-adenosyl-L-homocysteine + ferulic acid
-
?
S-adenosyl-L-methionine + caffeoyl alcohol
-
Triticum aestivum S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + caffeoyl aldehyde
-
Triticum aestivum S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + caffeoyl-CoA
-
Triticum aestivum S-adenosyl-L-homocysteine + feruloyl-CoA
-
?

Subunits

Subunits Comment Organism
? x * 38600, about, sequence calculation Triticum aestivum

Synonyms

Synonyms Comment Organism
caffeic acid 3-O-methyltransferase
-
Triticum aestivum
caffeic acid 3-O-methyltransferase
-
Nicotiana tabacum
COMT
-
Triticum aestivum
COMT
-
Nicotiana tabacum

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Triticum aestivum

General Information

General Information Comment Organism
metabolism COMT plays a pivotal position in the lignin biosynthetic pathway Triticum aestivum
metabolism COMT plays a pivotal position in the lignin biosynthetic pathway Nicotiana tabacum