Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Triticum aestivum |
recombinant expression in transgenic plants using the transformation by Agrobacterium tumefaciens strain LBA4404 | Nicotiana tabacum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of transgenic COMT knockout Nicotiana tabacum plants by antisense expression of COMT from Triticum aestivum via Agrobacterium tumefaciens transfection, phenotype, overview | Triticum aestivum |
additional information | construction of transgenic COMT knockout Nicotiana tabacum plants by antisense expression of COMT from Triticum aestivum, phenotype, overview | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0173 | - |
5-hydroxy-coniferaldehyde | - |
Triticum aestivum | |
0.0437 | - |
caffeoyl aldehyde | - |
Triticum aestivum | |
0.0688 | - |
caffeic acid | - |
Triticum aestivum | |
0.083 | - |
caffeoyl-CoA | - |
Triticum aestivum | |
0.0952 | - |
5-hydroxy-feruloyl-CoA | - |
Triticum aestivum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38600 | - |
x * 38600, about, sequence calculation | Triticum aestivum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Triticum aestivum | COMT catalyzes the multi-step methylation reactions of hydroxylated monomeric lignin precursors, and plays a pivotal position in the lignin biosynthetic pathway | ? | - |
? | |
additional information | Nicotiana tabacum | COMT catalyzes the multi-step methylation reactions of hydroxylated monomeric lignin precursors, and plays a pivotal position in the lignin biosynthetic pathway | ? | - |
? | |
S-adenosyl-L-methionine + caffeoyl-CoA | Triticum aestivum | - |
S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | O24149 | cv. Wisconsin 38 | - |
Triticum aestivum | Q38J50 | cv. H4564 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, expression of antisense enzyme in transgenic Nicotiana tabacum plants | Triticum aestivum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
determination of lignin content in transgenic plants, overview | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | COMT catalyzes the multi-step methylation reactions of hydroxylated monomeric lignin precursors, and plays a pivotal position in the lignin biosynthetic pathway | Triticum aestivum | ? | - |
? | |
additional information | COMT catalyzes the multi-step methylation reactions of hydroxylated monomeric lignin precursors, and plays a pivotal position in the lignin biosynthetic pathway | Nicotiana tabacum | ? | - |
? | |
additional information | the enzyme is capable of 3'- and 5'-methylating not only acid, aldehyde and alcohol precursors, but also ester precursors, broad substrate specificity, overview | Triticum aestivum | ? | - |
? | |
S-adenosyl-L-methionine + 5-hydroxy-feruloyl-CoA | - |
Triticum aestivum | S-adenosyl-L-homocysteine + sinapoyl-CoA | - |
? | |
S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde | - |
Triticum aestivum | S-adenosyl-L-homocysteine + sinapaldehyde | - |
? | |
S-adenosyl-L-methionine + caffeic acid | - |
Triticum aestivum | S-adenosyl-L-homocysteine + ferulic acid | - |
? | |
S-adenosyl-L-methionine + caffeoyl alcohol | - |
Triticum aestivum | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + caffeoyl aldehyde | - |
Triticum aestivum | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + caffeoyl-CoA | - |
Triticum aestivum | S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38600, about, sequence calculation | Triticum aestivum |
Synonyms | Comment | Organism |
---|---|---|
caffeic acid 3-O-methyltransferase | - |
Triticum aestivum |
caffeic acid 3-O-methyltransferase | - |
Nicotiana tabacum |
COMT | - |
Triticum aestivum |
COMT | - |
Nicotiana tabacum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Triticum aestivum |
General Information | Comment | Organism |
---|---|---|
metabolism | COMT plays a pivotal position in the lignin biosynthetic pathway | Triticum aestivum |
metabolism | COMT plays a pivotal position in the lignin biosynthetic pathway | Nicotiana tabacum |