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Literature summary for 2.1.1.104 extracted from

  • Wils, C.R.; Brandt, W.; Manke, K.; Vogt, T.
    A single amino acid determines position specificity of an Arabidopsis thaliana CCoAOMT-like O-methyltransferase (2013), FEBS Lett., 587, 683-689.
    View publication on PubMed
Search for more literature for 2.1.1.104

Cloned(Commentary)

Cloned (Comment) Organism
-
 Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
G46Y mutation is sufficient for a reversal of the unusual para- back to meta-O-methylation of flavanones and dihydroflavonols Arabidopsis thaliana
G46Y/W193F/R194M/I195K methylation in meta-position is preferred, mutations reduce the affinity for eriodictyol due to almost no interactions of the side chains with the substrate Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 eriodictyol mutant G46Y, pH 7.5, 37°C Arabidopsis thaliana
0.063
-
 eriodictyol wild-type, pH 7.5, 37°C Arabidopsis thaliana
0.28
-
 eriodictyol mutant G46Y/W193F/R194M/I195K, pH 7.5, 37°C Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9C5D7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme shows a strong preference for methylating the para position of flavanones and dihydroflavonols, whereas flavones and flavonols are methylated in the meta-position Arabidopsis thaliana ?
-
 ?
S-adenosyl-L-methionine + caffeic acid 10% of the activity with luteolin Arabidopsis thaliana S-adenosyl-L-homocysteine + ferulic acid synthesis of a single meta-methylated product ?
S-adenosyl-L-methionine + caffeoyl-CoA 33% of the activity with luteolin Arabidopsis thaliana S-adenosyl-L-homocysteine + feruloyl CoA
-
 ?
S-adenosyl-L-methionine + dihydroquercetin 2.5% of the activity with luteolin Arabidopsis thaliana S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + eriodictyol 15% of the activity with luteolin Arabidopsis thaliana S-adenosyl-L-homocysteine + eriodictoyl-3'-O-methylether + eriodictoyl-4'-O-methylether 80% para product versus 20% meta in the wild type ?
S-adenosyl-L-methionine + esculetin 20% of the activity with luteolin Arabidopsis thaliana S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + luteolin
-
 Arabidopsis thaliana S-adenosyl-L-homocysteine + scoparol synthesis of a single meta-methylated product ?
S-adenosyl-L-methionine + quercetin 60% of the activity with luteolin Arabidopsis thaliana S-adenosyl-L-homocysteine + isorhamnetin synthesis of a single meta-methylated product ?

Synonyms

Synonyms Comment Organism
At4g26220
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.023
-
 eriodictyol mutant G46Y, pH 7.5, 37°C Arabidopsis thaliana
0.029
-
 eriodictyol mutant G46Y/W193F/R194M/I195K, pH 7.5, 37°C Arabidopsis thaliana
0.08
-
 eriodictyol wild-type, pH 7.5, 37°C Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.102
-
 eriodictyol mutant G46Y/W193F/R194M/I195K, pH 7.5, 37°C Arabidopsis thaliana
0.92
-
 eriodictyol mutant G46Y, pH 7.5, 37°C Arabidopsis thaliana
1.3
-
 eriodictyol wild-type, pH 7.5, 37°C Arabidopsis thaliana