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Literature summary for 2.1.1.107 extracted from

  • Chou, P.L.; Ohtsuka, M.; Minowa, T.; Yamasato, K.; Sakano, Y.; Matsuzawa, H.; Ohta, T.; Sakai, H.
    Reddish Escherichia coli cells caused by overproduction of Bacillus stearothermophilus uroporphyrinogen III methylase: cloning, sequencing, and expression of the gene (1995), Biosci. Biotechnol. Biochem., 59, 1817-1824.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
in Escherichia coli JM83 and MV1184 Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 homodimer, 2 * 27000, SDS-PAGE, gel filtration Geobacillus stearothermophilus
27610
-
 calculated from DNA sequence Geobacillus stearothermophilus
28000
-
 SDS-PAGE Geobacillus stearothermophilus
50000
-
 gel filtration Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 S-adenosyl-L-methionine + uroporphyrinogen III Geobacillus stearothermophilus
-
 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from recombinant E. coli Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + uroporphyrinogen III
-
 Geobacillus stearothermophilus 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?

Subunits

Subunits Comment Organism
dimer homodimer, 2 * 27000, SDS-PAGE, gel filtration Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 thermostable, 17% of activity remaining after 10 min incubation Geobacillus stearothermophilus