Literature summary for 2.1.1.107 extracted from

Araya, M.A.; Tantalean, J.C.; Perez, J.M.; Fuentes, D.E.; Calderon, I.L.; Saavedra, C.P.; Burra, R.; Chasteen, T.G.; Vasquez, C.C.
Cloning, purification and characterization of Geobacillus stearothermophilus V uroporphyrinogen-III C-methyltransferase: evaluation of its role in resistance to potassium tellurite in Escherichia coli (2009), Res. Microbiol., 160, 125-133.

Search for more literature for 2.1.1.107

Cloned(Commentary)

Cloned (Comment)	Organism
gene cobA, expression in Escherichia coli strain JM109(DE3), the recombinant enzyme renders the bacteria morre resistant to potassium tellurite and potassium tellurate	Geobacillus stearothermophilus

Protein Variants

Protein Variants	Comment	Organism
G12A	site-directed mutagenesis of cobA abolishes the tellurite resistance of the mesophilic, heterologous host Escherichia coli and the SUMT activity in vitro. Cells overexpressing SUMT G12A show 7fold less tolerance to K2TeO3 as compared to that exhibit by cells expressing the wild-type methyltransferase	Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30000	-	2 * 30000, recombinant enzyme, SDS-PAGE	Geobacillus stearothermophilus
60000	-	recombinant enzyme, native PAGE and gel filtration	Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	Q6TA16	gene cobA	-
Geobacillus stearothermophilus V	Q6TA16	gene cobA	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
uroporphyrinogen III + S-adenosyl-L-methionine	the enzyme binds S-adenosyl-L-methionine and catalyzes the production of III methyl uroporphyrinogen in vitro	Geobacillus stearothermophilus	precorrin-2 + S-adenosyl-L-homocysteine	-	?
uroporphyrinogen III + S-adenosyl-L-methionine	the enzyme binds S-adenosyl-L-methionine and catalyzes the production of III methyl uroporphyrinogen in vitro	Geobacillus stearothermophilus V	precorrin-2 + S-adenosyl-L-homocysteine	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 30000, recombinant enzyme, SDS-PAGE	Geobacillus stearothermophilus

Synonyms

Synonyms	Comment	Organism
SUMT	-	Geobacillus stearothermophilus

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	-	Geobacillus stearothermophilus

General Information

General Information	Comment	Organism
physiological function	the role of SUMT methyltransferase in tellurite(ate) detoxification is not related to tellurium volatilization	Geobacillus stearothermophilus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)