Cloned (Comment) | Organism |
---|---|
gene mboIIR, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli using the constitutive promoter, PtetA | Moraxella bovis |
gene ncuIM2, DNA and amino acid sequence determination and analysis, expression in Escherichia coli | Moraxella cuniculi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Moraxella bovis | |
Ca2+ | slight inhibition | Moraxella cuniculi | |
Mg2+ | - |
Moraxella bovis | |
Mg2+ | slight inhibition | Moraxella cuniculi | |
Mn2+ | - |
Moraxella bovis | |
Mn2+ | strong inhibition | Moraxella cuniculi | |
Zn2+ | - |
Moraxella bovis | |
Zn2+ | strong inhibition | Moraxella cuniculi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
1 * 30000, about, recombinant enzyme, 1 * 32676, sequence calculation | Moraxella cuniculi |
30000 | - |
1 * 30000, about, recombinant enzyme, SDS-PAGE, 1 * 32111, sequence calculation | Moraxella bovis |
32111 | - |
1 * 30000, about, recombinant enzyme, SDS-PAGE, 1 * 32111, sequence calculation | Moraxella bovis |
32676 | - |
1 * 30000, about, recombinant enzyme, 1 * 32676, sequence calculation | Moraxella cuniculi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + [DNA]-cytosine | Moraxella bovis | - |
S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine | - |
? | |
S-adenosyl-L-methionine + [DNA]-cytosine | Moraxella cuniculi | - |
S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moraxella bovis | - |
strain ATCC 10900, gene mboIIR | - |
Moraxella cuniculi | - |
strain ATCC 14688, gene ncuIM2 | - |
Purification (Comment) | Organism |
---|---|
recombinant M2.MboII 3.6fold to electrophoretic homogeneity using a four-step chromatographic procedure, involving adsorption and hydroxylapatite chromatography followed by hydroophobic interaction and heparin affinity chromatography, from Escherichia coli | Moraxella bovis |
recombinant M2.NcuI 3.6fold to electrophoretic homogeneity using a four-step chromatographic procedure, involving adsorption and hydroxylapatite chromatography followed by hydrophobic interaction and heparin affinity chromatography, from Escherichia coli | Moraxella cuniculi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3200 | - |
purified recombinant enzyme | Moraxella bovis |
3200 | - |
purified recombinant enzyme | Moraxella cuniculi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + [DNA]-cytosine | - |
Moraxella bovis | S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine | - |
? | |
S-adenosyl-L-methionine + [DNA]-cytosine | - |
Moraxella cuniculi | S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine | - |
? | |
S-adenosyl-L-methionine + [DNA]-cytosine | substrate is pNH20 plasmid DNA. M2.MboII modifies the internal cytosine in the recognition sequence 39-CTTCT-59, yielding N4-methylcytosine, and moreover is able to methylate double- and single-stranded DNA, single-stranded DNA is preferred. Determination and analysis of the methylation pattern of M2.MboII, overview | Moraxella bovis | S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine | - |
? | |
S-adenosyl-L-methionine + [DNA]-cytosine | substrate is pNH20 plasmid DNA. M2.NcuI modifies the internal cytosine in the recognition sequence yielding N4-methylcytosine, and moreover is able to methylate double- and single-stranded DNA, single-stranded DNA is preferred. Determination of the methylation position, overview | Moraxella cuniculi | S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, about, recombinant enzyme, 1 * 32676, sequence calculation | Moraxella cuniculi |
monomer | 1 * 30000, about, recombinant enzyme, SDS-PAGE, 1 * 32111, sequence calculation | Moraxella bovis |
Synonyms | Comment | Organism |
---|---|---|
M2.MboII | - |
Moraxella bovis |
M2.NcuI | - |
Moraxella cuniculi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Moraxella bovis |
37 | - |
assay at | Moraxella cuniculi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Moraxella bovis |
7 | - |
assay at | Moraxella cuniculi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Moraxella bovis | |
S-adenosyl-L-methionine | - |
Moraxella cuniculi |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.4 | - |
pH 7.0, 37°C, recombinant enzyme | Moraxella bovis | Mn2+ | |
0.4 | - |
pH 7.0, 37°C, recombinant enzyme | Moraxella bovis | Zn2+ | |
4 | - |
pH 7.0, 37°C, recombinant enzyme | Moraxella bovis | Ca2+ | |
5 | - |
pH 7.0, 37°C, recombinant enzyme | Moraxella bovis | Mg2+ |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is part of the MboII restriction-modification, R-M, system of Moraxella bovis strain ATCC 10900 consisting of a restriction endonuclease gene and two methyltransferase genes | Moraxella bovis |
metabolism | the enzyme is part of the Ncul restriction-modification, R-M, system consisting of a restriction endonuclease gene and two methyltransferase genes | Moraxella cuniculi |