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Literature summary for 2.1.1.113 extracted from

  • Furmanek-Blaszk, B.; Boratynski, R.; Zolcinska, N.; Sektas, M.
    M1.Mboll and M2.Mboll type IIS methyltransferases: different specificities, the same target (2009), Microbiology, 155, 1111-1121.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene mboIIR, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli using the constitutive promoter, PtetA Moraxella bovis
gene ncuIM2, DNA and amino acid sequence determination and analysis, expression in Escherichia coli Moraxella cuniculi

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Moraxella bovis
Ca2+ slight inhibition Moraxella cuniculi
Mg2+
-
Moraxella bovis
Mg2+ slight inhibition Moraxella cuniculi
Mn2+
-
Moraxella bovis
Mn2+ strong inhibition Moraxella cuniculi
Zn2+
-
Moraxella bovis
Zn2+ strong inhibition Moraxella cuniculi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
1 * 30000, about, recombinant enzyme, 1 * 32676, sequence calculation Moraxella cuniculi
30000
-
1 * 30000, about, recombinant enzyme, SDS-PAGE, 1 * 32111, sequence calculation Moraxella bovis
32111
-
1 * 30000, about, recombinant enzyme, SDS-PAGE, 1 * 32111, sequence calculation Moraxella bovis
32676
-
1 * 30000, about, recombinant enzyme, 1 * 32676, sequence calculation Moraxella cuniculi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + [DNA]-cytosine Moraxella bovis
-
S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine
-
?
S-adenosyl-L-methionine + [DNA]-cytosine Moraxella cuniculi
-
S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine
-
?

Organism

Organism UniProt Comment Textmining
Moraxella bovis
-
strain ATCC 10900, gene mboIIR
-
Moraxella cuniculi
-
strain ATCC 14688, gene ncuIM2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant M2.MboII 3.6fold to electrophoretic homogeneity using a four-step chromatographic procedure, involving adsorption and hydroxylapatite chromatography followed by hydroophobic interaction and heparin affinity chromatography, from Escherichia coli Moraxella bovis
recombinant M2.NcuI 3.6fold to electrophoretic homogeneity using a four-step chromatographic procedure, involving adsorption and hydroxylapatite chromatography followed by hydrophobic interaction and heparin affinity chromatography, from Escherichia coli Moraxella cuniculi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3200
-
purified recombinant enzyme Moraxella bovis
3200
-
purified recombinant enzyme Moraxella cuniculi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + [DNA]-cytosine
-
Moraxella bovis S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine
-
?
S-adenosyl-L-methionine + [DNA]-cytosine
-
Moraxella cuniculi S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine
-
?
S-adenosyl-L-methionine + [DNA]-cytosine substrate is pNH20 plasmid DNA. M2.MboII modifies the internal cytosine in the recognition sequence 39-CTTCT-59, yielding N4-methylcytosine, and moreover is able to methylate double- and single-stranded DNA, single-stranded DNA is preferred. Determination and analysis of the methylation pattern of M2.MboII, overview Moraxella bovis S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine
-
?
S-adenosyl-L-methionine + [DNA]-cytosine substrate is pNH20 plasmid DNA. M2.NcuI modifies the internal cytosine in the recognition sequence yielding N4-methylcytosine, and moreover is able to methylate double- and single-stranded DNA, single-stranded DNA is preferred. Determination of the methylation position, overview Moraxella cuniculi S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine
-
?

Subunits

Subunits Comment Organism
monomer 1 * 30000, about, recombinant enzyme, 1 * 32676, sequence calculation Moraxella cuniculi
monomer 1 * 30000, about, recombinant enzyme, SDS-PAGE, 1 * 32111, sequence calculation Moraxella bovis

Synonyms

Synonyms Comment Organism
M2.MboII
-
Moraxella bovis
M2.NcuI
-
Moraxella cuniculi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Moraxella bovis
37
-
assay at Moraxella cuniculi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
Moraxella bovis
7
-
assay at Moraxella cuniculi

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Moraxella bovis
S-adenosyl-L-methionine
-
Moraxella cuniculi

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.4
-
pH 7.0, 37°C, recombinant enzyme Moraxella bovis Mn2+
0.4
-
pH 7.0, 37°C, recombinant enzyme Moraxella bovis Zn2+
4
-
pH 7.0, 37°C, recombinant enzyme Moraxella bovis Ca2+
5
-
pH 7.0, 37°C, recombinant enzyme Moraxella bovis Mg2+

General Information

General Information Comment Organism
metabolism the enzyme is part of the MboII restriction-modification, R-M, system of Moraxella bovis strain ATCC 10900 consisting of a restriction endonuclease gene and two methyltransferase genes Moraxella bovis
metabolism the enzyme is part of the Ncul restriction-modification, R-M, system consisting of a restriction endonuclease gene and two methyltransferase genes Moraxella cuniculi