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Literature summary for 2.1.1.117 extracted from

  • Sato, F.; Takeshita, N.; Fitchen, J.H.; Fujiwara, H.; Yamada, Y.
    S-Adenosyl-L-methionine:scoulerine-9-O-methyltransferase from cultured Coptis japonica cells (1993), Phytochemistry, 32, 659-664.
No PubMed abstract available
Search for more literature for 2.1.1.117

Inhibitors

Inhibitors Comment Organism Structure
Berberine 5 mM, 70% inhibition Coptis japonica
Ca2+ 1 mM, 64% inhibition Coptis japonica
Cu2+ 1 mM, 72% inhibition Coptis japonica
Mn2+ 1 mM, 59% inhibition Coptis japonica
palmatine 5 mM, 70% inhibition Coptis japonica
PCMB 0.5 mM, 51% inhibition Coptis japonica
S-adenosylhomocysteine
-
 Coptis japonica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 (S)-scoulerine
-
 Coptis japonica
0.17
-
 S-adenosyl-L-methionine
-
 Coptis japonica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 x * 41000, SDS-PAGE Coptis japonica
120000 140000 gel filtration Coptis japonica

Organism

Organism UniProt Comment Textmining
Coptis japonica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Coptis japonica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-Adenosyl-L-methionine + (S)-scoulerine
-
 Coptis japonica S-Adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
-
 ?
S-Adenosyl-L-methionine + 6-O-methylnorlaudanosoline 8% of the activity relative to (S)-scoulerine Coptis japonica ?
-
 ?
S-Adenosyl-L-methionine + norlaudanosoline 2% of the activity relative to (S)-scoulerine Coptis japonica ?
-
 ?
S-Adenosyl-L-methionine + norreticuline 9% of the activity relative to (S)-scoulerine Coptis japonica ?
-
 ?

Subunits

Subunits Comment Organism
? x * 41000, SDS-PAGE Coptis japonica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 9.5
-
 Coptis japonica

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10 50% of maximal activity at pH 7.5 and at pH 10 Coptis japonica