KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
L-homocysteine | - |
Phaseolus vulgaris | |
2.4 | - |
N5-methyltetrahydropteroylmonoglutamate | - |
Phaseolus vulgaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Phaseolus vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
gel filtration | Phaseolus vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaseolus vulgaris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Phaseolus vulgaris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
assay method | Phaseolus vulgaris |
Storage Stability | Organism |
---|---|
0°C, pH 7, half-life 6 days | Phaseolus vulgaris |
freeze-dried, 20% loss of activity after 3 months | Phaseolus vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N5-methyltetrahydropteroylmonoglutamate + L-homocysteine | - |
Phaseolus vulgaris | tetrahydropteroylmonoglutamate + L-methionine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
31 | - |
- |
Phaseolus vulgaris |
39 | - |
- |
Phaseolus vulgaris |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 39 | 10°C: about 10% of maximum activity, 39°C: maximum activity | Phaseolus vulgaris |
15 | 31 | 15°C: about 10% of maximum activity, 31°C: maximum activity | Phaseolus vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Phaseolus vulgaris |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
stability optimum, 0°C, t1/2: 6 days | Phaseolus vulgaris |