Cloned (Comment) | Organism |
---|---|
expressed as a GST fusion protein in Saccharomyces cerevisiae strain SDYalpha | Candida albicans |
Protein Variants | Comment | Organism |
---|---|---|
D504A | the mutant exhibits 4% of wild type activity | Candida albicans |
D504N | the mutant exhibits 11% of wild type activity | Candida albicans |
D504S | the mutant exhibits 7% of wild type activity | Candida albicans |
D614A | the mutant exhibits less than 2% of wild type activity | Candida albicans |
D614N | the mutant exhibits less than 2% of wild type activity | Candida albicans |
D614S | the mutant exhibits 11% of wild type activity | Candida albicans |
E620Q | the mutation has no effect on enzyme activity | Candida albicans |
E620S | the mutation has no effect on enzyme activity | Candida albicans |
H128N | the mutant is 5fold less active compared to the wild type GST fusion protein | Candida albicans |
R530A | the mutant exhibits 33% of wild type activity | Candida albicans |
S448A | the mutant exhibits 67% of wild type activity | Candida albicans |
W576F | the mutant is 3fold less active compared to the wild type GST fusion protein | Candida albicans |
W576Y | the mutant is 8fold less active compared to the wild type GST fusion protein | Candida albicans |
Y527F | the mutant exhibits 67% of wild type activity | Candida albicans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
L-homocysteine | in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-HCl (pH 7.2), 0.1 mM MgSO4, and 10 mM dithiothreitol, at 37°C | Candida albicans | |
0.165 | - |
N5-methyltetrahydropteroylmonoglutamate | in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-HCl (pH 7.2), 0.1 mM MgSO4, and 10 mM dithiothreitol, at 37°C | Candida albicans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
86000 | - |
SDS-PAGE | Candida albicans |
112000 | - |
GST fusion protein, SDS-PAGE | Candida albicans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione agarose column chromatography | Candida albicans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N5-methyltetrahydropteroylmonoglutamate + L-homocysteine | - |
Candida albicans | tetrahydropteroylmonoglutamate + L-methionine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Met6p | - |
Candida albicans |