Cloned (Comment) | Organism |
---|---|
mutant enzymes I690C/G743C and I690C/G743C/Y1139 are expressed in Escherichia coli Hms174(DE3) cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
I690C/G743C | the variant of a C-terminal fragment of MetH locks the enzyme into the activation conformation without perturbing any of the residues in the vicinity of the active site | Escherichia coli |
I690C/G743C/Y1139F | the I690C/G743C MetH variant is trapped in the activation conformation | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Hi-Trap column chromatography and MonoQ column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N5-methyltetrahydropteroylmonoglutamate + L-homocysteine | - |
Escherichia coli | tetrahydropteroylmonoglutamate + L-methionine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MetH | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
vitamin B12 | dependent on | Escherichia coli |