Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) pLysS cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C271S | the mutation does not affect the activity and structure of the enzyme | Homo sapiens |
C334S | the mutation decreases the enzymatic turnover and changes the conformation of the enzyme | Homo sapiens |
C360S | the mutation decreases the enzymatic turnover and changes the conformation of the enzyme | Homo sapiens |
C375S | the mutation does not affect the activity and structure of the enzyme | Homo sapiens |
C72S | the mutant is completely inactive | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
arsenite | mutant enzyme C334S, in PBS (25 mM, pH 7.0), at 37°C | Homo sapiens | |
0.00188 | - |
arsenite | mutant enzyme C271S, in PBS (25 mM, pH 7.0), at 37°C | Homo sapiens | |
0.00232 | - |
arsenite | mutant enzyme C360S, in PBS (25 mM, pH 7.0), at 37°C | Homo sapiens | |
0.0032 | - |
arsenite | wild type enzyme, in PBS (25 mM, pH 7.0), at 37°C | Homo sapiens | |
0.0185 | - |
arsenite | mutant enzyme C375S, in PBS (25 mM, pH 7.0), at 37°C | Homo sapiens | |
0.0478 | - |
S-adenosyl-L-methionine | wild type enzyme, in PBS (25 mM, pH 7.0), at 37°C | Homo sapiens | |
0.049 | - |
S-adenosyl-L-methionine | mutant enzyme C375S, in PBS (25 mM, pH 7.0), at 37°C | Homo sapiens | |
0.0495 | - |
S-adenosyl-L-methionine | mutant enzyme C360S, in PBS (25 mM, pH 7.0), at 37°C | Homo sapiens | |
0.0503 | - |
S-adenosyl-L-methionine | mutant enzyme C334S, in PBS (25 mM, pH 7.0), at 37°C | Homo sapiens | |
0.0512 | - |
S-adenosyl-L-methionine | mutant enzyme C271S, in PBS (25 mM, pH 7.0), at 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + arsenite | - |
Homo sapiens | S-adenosyl-L-homocysteine + methylarsonate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
arsenic (+3 oxidation state) methyltransferase | - |
Homo sapiens |
AS3MT | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
the wild type enzyme exhibits about 40% residual activity after 120 min at 45°C | Homo sapiens |