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Literature summary for 2.1.1.14 extracted from

  • Eckermann, C.; Eichel, J.; Schroder, J.
    Plant methionine synthase: new insights into properties and expression (2000), Biol. Chem., 381, 695-703.
    View publication on PubMed
Search for more literature for 2.1.1.14

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate
-
 Catharanthus roseus
0.35
-
 5-methyltetrahydropteroylmonoglutamate
-
 Catharanthus roseus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ no stimulation Catharanthus roseus
phosphate
-
 Catharanthus roseus
Zn2+
-
 Catharanthus roseus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
 gel filtration, recombinant enzyme Catharanthus roseus

Organism

Organism UniProt Comment Textmining
Catharanthus roseus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Catharanthus roseus
-
cotyledon
-
 Catharanthus roseus
-
hypocotyl
-
 Catharanthus roseus
-
seedling low activity Catharanthus roseus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
 Catharanthus roseus tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
 ?
5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
 Catharanthus roseus tetrahydropteroylmonoglutamate + L-methionine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 85000, SDS-PAGE, recombinant enzyme Catharanthus roseus