Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, 80% loss of activity | Arabidopsis thaliana | |
Cu2+ | 5 mM, 80% loss of activity | Arabidopsis thaliana | |
Fe2+ | 5 mM, 80% loss of activity | Arabidopsis thaliana | |
Mg2+ | 5 mM, 80% loss of activity | Arabidopsis thaliana | |
Mn2+ | 5 mM, 80% loss of activity | Arabidopsis thaliana | |
Na+ | 5 mM, 50-60% inhibition | Arabidopsis thaliana | |
NH4+ | 5 mM, 50-60% inhibition | Arabidopsis thaliana | |
Zn2+ | 5 mM, 80% loss of activity | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0063 | - |
S-adenosyl-L-methionine | pH 7.5, 20°C | Arabidopsis thaliana | |
0.0385 | - |
jasmonate | pH 7.5, 20°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Arabidopsis thaliana | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | highest activity in presence of 100 mM KCl | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + jasmonate | Arabidopsis thaliana | expression of the gene is induced both locally and systematically by wounding or methyl jasmonate treatement. The jasmonic acid carboxyl methyltransferase is a key enzyme for jasmonate-regulated plant responses. Activation of JMT expression leads to production of methyl jasmonate that could act as an intracellular regulator, a diffusible intercellular transducer, and an airborne signal mediating intra- and interplant communications | S-adenosyl-L-homocysteine + methyl jasmonate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9AR07 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme does not convert 12-oxo-phytodienoic acid, a precursor of jasmonic acid, salicylic acid, benzoic acid, linolenic acid or cinnamic acid into their corresponding methyl esters | Arabidopsis thaliana | ? | - |
? | |
S-adenosyl-L-methionine + 9,10-dihydrojasmonic acid | poor substrate | Arabidopsis thaliana | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + jasmonate | - |
Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl jasmonate | - |
? | |
S-adenosyl-L-methionine + jasmonate | expression of the gene is induced both locally and systematically by wounding or methyl jasmonate treatement. The jasmonic acid carboxyl methyltransferase is a key enzyme for jasmonate-regulated plant responses. Activation of JMT expression leads to production of methyl jasmonate that could act as an intracellular regulator, a diffusible intercellular transducer, and an airborne signal mediating intra- and interplant communications | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl jasmonate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
25 | - |
jasmonate | pH 7.5, 20°C | Arabidopsis thaliana | |
70 | - |
S-adenosyl-L-methionine | pH 7.5, 20°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Arabidopsis thaliana |