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Literature summary for 2.1.1.141 extracted from

  • Seo, H.S.; Song, J.T.; Cheong, J.J.; Lee, Y.H.; Lee, Y.W.; Hwang, I.; Lee, J.S.; Choi, Y.D.
    Jasmonic acid carboxyl methyltransferase: A key enzyme for jasmonate-regulated plant responses (2001), Proc. Natl. Acad. Sci. USA, 98, 4788-4793.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 5 mM, 80% loss of activity Arabidopsis thaliana
Cu2+ 5 mM, 80% loss of activity Arabidopsis thaliana
Fe2+ 5 mM, 80% loss of activity Arabidopsis thaliana
Mg2+ 5 mM, 80% loss of activity Arabidopsis thaliana
Mn2+ 5 mM, 80% loss of activity Arabidopsis thaliana
Na+ 5 mM, 50-60% inhibition Arabidopsis thaliana
NH4+ 5 mM, 50-60% inhibition Arabidopsis thaliana
Zn2+ 5 mM, 80% loss of activity Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0063
-
S-adenosyl-L-methionine pH 7.5, 20°C Arabidopsis thaliana
0.0385
-
jasmonate pH 7.5, 20°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Arabidopsis thaliana 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
KCl highest activity in presence of 100 mM KCl Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + jasmonate Arabidopsis thaliana expression of the gene is induced both locally and systematically by wounding or methyl jasmonate treatement. The jasmonic acid carboxyl methyltransferase is a key enzyme for jasmonate-regulated plant responses. Activation of JMT expression leads to production of methyl jasmonate that could act as an intracellular regulator, a diffusible intercellular transducer, and an airborne signal mediating intra- and interplant communications S-adenosyl-L-homocysteine + methyl jasmonate
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9AR07
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme does not convert 12-oxo-phytodienoic acid, a precursor of jasmonic acid, salicylic acid, benzoic acid, linolenic acid or cinnamic acid into their corresponding methyl esters Arabidopsis thaliana ?
-
?
S-adenosyl-L-methionine + 9,10-dihydrojasmonic acid poor substrate Arabidopsis thaliana S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + jasmonate
-
Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl jasmonate
-
?
S-adenosyl-L-methionine + jasmonate expression of the gene is induced both locally and systematically by wounding or methyl jasmonate treatement. The jasmonic acid carboxyl methyltransferase is a key enzyme for jasmonate-regulated plant responses. Activation of JMT expression leads to production of methyl jasmonate that could act as an intracellular regulator, a diffusible intercellular transducer, and an airborne signal mediating intra- and interplant communications Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl jasmonate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
25
-
jasmonate pH 7.5, 20°C Arabidopsis thaliana
70
-
S-adenosyl-L-methionine pH 7.5, 20°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
Arabidopsis thaliana