Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli, recombinant protein | Glycine max |
expression in Escherichia coli | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
24,25-dehydro-27-methenyl-cycloartenol | i.e. cycloartenol nucleus substrate analogue with double bond at C8 | Glycine max | |
24,25-dehydro-27-methylene-cycloartenol | i.e. cycloartenol nucleus substrate analogue with double bond at C14, non-competitive | Glycine max | |
24-dehydrocycloartenol | a mechanism-based inactivator | Glycine max | |
24-thiacycloartanol | a mechanism-based inactivator | Glycine max | |
26-methenylcycloartenol | pseudo-first-order time-dependent inactivation, less potency of inhibition | Glycine max | |
26-methynylcycloartenol | inhibition kinetics non-competitive and time-dependent, less potency of inhibition | Glycine max | |
Cycloartenol | - |
Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, overview | Glycine max | |
0.005 | - |
24,25-dehydro-27-methylene-cycloartenol | pH 7.5, 35°C, recombinant enzyme | Glycine max | |
0.03 | - |
Cycloartenol | pH 7.5, 35°C, recombinant enzyme | Glycine max |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cycloartenol + S-adenosyl-L-methionine | Glycine max | - |
(24S)-24-methylcycloart-25-en-3beta-ol + S-adenosyl-L-homocysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially purified, recombinant protein, SDS-PAGE | Glycine max |
recombinant enzyme from Escherichia coli to homogeneity | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture supernatant | - |
Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
C-methylation reaction analyzed with substrate analogs containing a cycloartenol nucleus and a double bond or triple bond attached to C26, mass spectra of enzyme-generated products indicated, time-dependent inhibition kinetics reveals active-site directed process and partitioning to produce novel products | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
24,25-dehydro-27-methenyl-cycloartenol + S-adenosyl-L-methionine | CA-8, i.e. cycloartenol nucleus substrate analogue with double bond at C8, an enzyme inhibitor, is a poor substrate | Glycine max | ? | - |
? | |
24,25-dehydro-27-methylene-cycloartenol + S-adenosyl-L-methionine | CA-14, i.e. cycloartenol nucleus substrate analogue with double bond at C14, an enzyme inhibitor, is a poor substrate | Glycine max | ? | - |
? | |
cycloartenol + S-adenosyl-L-methionine | - |
Glycine max | (24S)-24-methylcycloart-25-en-3beta-ol + S-adenosyl-L-homocysteine | - |
? | |
additional information | the SMT from soybean plants is bifunctional, performing methyl transferations to C24 and to C28 of the substrate, the enzyme also converts the inhibitor 24,25-dehydro-27-methenyl-cycloartenol, i.e. cycloartenol nucleus substrate analogue with double bond at C8, to a 24,27-dimethenyl-cycloartenol, CA-11, compound, overview | Glycine max | ? | - |
? | |
S-adenosyl-L-methionine + cycloartenol | active-site topography and reaction mechanisms analyzed with substrate analogs | Glycine max | S-adenosyl-L-homocysteine + 24-methylenecycloartanol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to a family of sterol 24-C-methyltransferases that perform distinct C1-activities, two different alkylations at C24 or C28, alkylation of the latter affects membrane fluidity | Glycine max |
SMT | - |
Glycine max |
SMT protein | - |
Glycine max |
sterol 24-C methyltransferase | - |
Glycine max |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Glycine max |
35 | - |
inhibition assay at | Glycine max |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000083 | - |
24,25-dehydro-27-methylene-cycloartenol | pH 7.5, 35°C, recombinant enzyme | Glycine max | |
0.01 | - |
Cycloartenol | pH 7.5, 35°C, recombinant enzyme | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Glycine max |
7.5 | - |
inhibition assay at | Glycine max |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Glycine max |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics, overview | Glycine max | |
0.002 | - |
24-thiacycloartanol | pH 7.5, 35°C, recombinant enzyme | Glycine max | |
0.03 | - |
Cycloartenol | natural substrate | Glycine max | |
0.042 | - |
24-dehydrocycloartenol | pH 7.5, 35°C, recombinant enzyme | Glycine max | |
0.047 | - |
24,25-dehydro-27-methenyl-cycloartenol | pH 7.5, 35°C, recombinant enzyme | Glycine max | |
0.047 | - |
26-methenylcycloartenol | rate of inactivation saturable, comparable to values obtained for cycloartenol | Glycine max | |
0.067 | - |
24,25-dehydro-27-methylene-cycloartenol | pH 7.5, 35°C, recombinant enzyme | Glycine max |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.067 | - |
co-incubation with 50 microM or 100 microM cycloartenol affords protection against inactivation generating 25% and 45% C-methylation | Glycine max | 26-methynylcycloartenol |