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Literature summary for 2.1.1.143 extracted from

  • Neelakandan, A.K.; Song, Z.; Wang, J.; Richards, M.H.; Wu, X.; Valliyodan, B.; Nguyen, H.T.; Nes, W.D.
    Cloning, functional expression and phylogenetic analysis of plant sterol 24C-methyltransferases involved in sitosterol biosynthesis (2009), Phytochemistry, 70, 1982-1998.
    View publication on PubMed
Search for more literature for 2.1.1.143

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Glycine max

Inhibitors

Inhibitors Comment Organism Structure
24(28)-methylene cycloartanol
-
 Glycine max
25-Azacycloartenol
-
 Glycine max
sitosterol
-
 Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
 Cycloartenol pH 7.5, 35°C Glycine max
0.028
-
 24(28)-methylene lophenol pH 7.5, 35°C Glycine max
0.03
-
 24(28)-methylene lophenol pH 7.5, 35°C Glycine max
0.035
-
 Cycloartenol pH 7.5, 35°C Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 4 * 40400, calculated, 4 * 40000, SDS-PAGE Glycine max
40300
-
 x * 40300, calculated Glycine max
40400
-
 4 * 40400, calculated, 4 * 40000, SDS-PAGE Glycine max
160000
-
 gel filtration Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max D2D5G3 isoform SMT2-1
-
Glycine max D2D5G4 isoform SMT2-2
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Glycine max
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 24(28)-methylene lophenol best substrate Glycine max S-adenosyl-L-homocysteine + 24(28)-ethylidene lophenol
-
 ?
S-adenosyl-L-methionine + 4alpha-methyl zymosterol 60% of the effectiveness of 24(28)-methylene lophenol Glycine max S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + cycloartenol
-
 Glycine max S-adenosyl-L-homocysteine + 24-methylenecycloartanol
-
 ?
S-adenosyl-L-methionine + ergosta-7,24(28)-dienol equally effective as 24(28)-methylene lophenol Glycine max S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + fecosterol equally effective as 24(28)-methylene lophenol Glycine max S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + zymosterol 70% of the effectiveness of 24(28)-methylene lophenol Glycine max S-adenosyl-L-homocysteine + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 40300, calculated Glycine max
tetramer 4 * 40400, calculated, 4 * 40000, SDS-PAGE Glycine max

Synonyms

Synonyms Comment Organism
sterol 24C-methyltransferase
-
 Glycine max

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.013
-
 24(28)-methylene lophenol pH 7.5, 35°C Glycine max
0.022
-
 24(28)-methylene lophenol pH 7.5, 35°C Glycine max

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00001
-
 25-Azacycloartenol substrate cycloartenol, pH 7.5, 35°C Glycine max
0.00002
-
 25-Azacycloartenol substrate 24(28)-methylene lophenol, pH 7.5, 35°C Glycine max
0.00002
-
 25-Azacycloartenol substrate cycloartenol, pH 7.5, 35°C Glycine max
0.077
-
 24(28)-methylene cycloartanol substrate cycloartenol, pH 7.5, 35°C Glycine max
0.1
-
 sitosterol substrate cycloartenol, pH 7.5, 35°C Glycine max
0.116
-
 24(28)-methylene cycloartanol substrate 24(28)-methylene lophenol, pH 7.5, 35°C Glycine max
0.154
-
 sitosterol substrate 24(28)-methylene lophenol, pH 7.5, 35°C Glycine max
0.2
-
 sitosterol substrate cycloartenol, pH 7.5, 35°C Glycine max
0.25
-
 24(28)-methylene cycloartanol substrate cycloartenol, pH 7.5, 35°C Glycine max
0.297
-
 sitosterol substrate 24(28)-methylene lophenol, pH 7.5, 35°C Glycine max

pI Value

Organism Comment pI Value Maximum pI Value
Glycine max calculated
-
 6.3

General Information

General Information Comment Organism
physiological function SMT isoform expression determines specific C24-methyl to C24-ethyl ratios to flowering whereas with seed development there is a disconnection such that the SMT transcript levels decrease against an increase in sterol content. Generally SMT2-2 is expressed more than SMT2-1 or SMT1 Glycine max