Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Paramecium bursaria Chlorella virus-1 |
Protein Variants | Comment | Organism |
---|---|---|
E190G | no detectable activity | Paramecium bursaria Chlorella virus-1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
10-methyl-5,8-dideazafolate | 0.2 mM, 90% inhibition | Paramecium bursaria Chlorella virus-1 | |
10-propargylfolate | 0.2 mM, 85% inhibition | Paramecium bursaria Chlorella virus-1 | |
5-fluorodeoxyuridine | 0.05 mM, 95% inhibition | Paramecium bursaria Chlorella virus-1 | |
8-aminopurinone deoxyribonucleoside 5'-phosphate | 0.05 mM, 50% inhibition | Paramecium bursaria Chlorella virus-1 | |
additional information | no inhibition by 10-propargyl-5,6-dideazafolate, 10-methylfolate, 8-azapurinone deoxyribonucleoside 5-phosphate | Paramecium bursaria Chlorella virus-1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
dUMP | - |
Paramecium bursaria Chlorella virus-1 | |
0.02 | - |
5,10-methylenetetrahydrofolate | - |
Paramecium bursaria Chlorella virus-1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
x * 26000, SDS-PAGE | Paramecium bursaria Chlorella virus-1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paramecium bursaria Chlorella virus-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Paramecium bursaria Chlorella virus-1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Paramecium bursaria Chlorella virus-1 | dTMP + tetrahydrofolate + FAD | - |
? | |
additional information | oxidation of NAD(P)H is linked to the reduction of enzyme bound FAD, dUMP is required for efficient FAD reduction, omitting of 5,10-methylenetetrahydrofolate from reaction mixture increases oxidation activity by a factor of 7 | Paramecium bursaria Chlorella virus-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26000, SDS-PAGE | Paramecium bursaria Chlorella virus-1 |
Synonyms | Comment | Organism |
---|---|---|
thymidylate synthase ThyX | - |
Paramecium bursaria Chlorella virus-1 |
ThyX | - |
Paramecium bursaria Chlorella virus-1 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | noncovalently bound | Paramecium bursaria Chlorella virus-1 | |
NADH | oxidation of NADH is linked to the reduction of enzyme bound FAD, dUMP is required for efficient FAD reduction, omitting of 5,10-methylenetetrahydrofolate from reaction mixture increases oxidation activity by a factor of 7 | Paramecium bursaria Chlorella virus-1 | |
NADPH | oxidation of NADPH is linked to the reduction of enzyme bound FAD, dUMP is required for efficient FAD reduction, omitting of 5,10-methylenetetrahydrofolate from reaction mixture increases oxidation activity by a factor of 7 | Paramecium bursaria Chlorella virus-1 |