Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Triticum aestivum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Triticum aestivum | Q38J50 | cv. Norstar | - |
Purification (Comment) | Organism |
---|---|
- |
Triticum aestivum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Triticum aestivum | - |
Storage Stability | Organism |
---|---|
4°C, the activity of the affinity-purified TaOMT2 is quite stable for up to 2 months when stored in buffer containing 10% glycerol, with a half-life of 10 to 11 weeks | Triticum aestivum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 S-adenosyl-L-methionine + tricetin | the enzyme catalyzes three sequential O-methylations, forming 3'-O-methyltricetin, 3',5'-dimethyltricetin and 3',4',5'-O-trimethyltricetin. Tricetin is the preferred substrate. Also catalyzes the methylation of 5-hydroxyferulic acid, luteolin, quercetin, eriodyctiol, quercetagetin, taxifolin, gossypetin and myricetin. TaOMT2 is a flavonoid, B-ring-specific O-methyltransferase with a preference for flavones > dihydroflavones > flavonols that possess at least two, B-ring hydroxyl groups | Triticum aestivum | 3 S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin | - |
? | |
S-adenosyl-L-methionine + 3',5'-O-dimethyltricetin | the enzyme catalyzes three sequential O-methylations, forming 3'-O-methyltricetin, 3',5'-dimethyltricetin and 3',4',5'-O-trimethyltricetin. Tricetin is the preferred substrate | Triticum aestivum | S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin | - |
? | |
S-adenosyl-L-methionine + 3'-O-methyltricetin | the enzyme catalyzes three sequential O-methylations, forming 3'-O-methyltricetin, 3',5'-dimethyltricetin and 3',4',5'-O-trimethyltricetin. Tricetin is the preferred substrate | Triticum aestivum | S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin | - |
? | |
S-adenosyl-L-methionine + tricetin | the enzyme catalyzes three sequential O-methylations, forming 3'-O-methyltricetin, 3',5'-dimethyltricetin and 3',4',5'-O-trimethyltricetin. Tricetin is the preferred substrate | Triticum aestivum | S-adenosyl-L-homocysteine + 3'-O-methyltricetin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TaOMT2 | - |
Triticum aestivum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Triticum aestivum |