Cloned (Comment) | Organism |
---|---|
gene rsmD, expression of N-terminally His6-tagged RsmD | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D58A | site-directed mutagenesis, the mutant shows increased Km toward the 30S subunit by two orders of magnitude with only a marginal effect on kcat compared to the wild-type enzyme. The mutation decreases the enthalpic contribution to SAM binding, paralleled by an increase in the entropic contribution | Escherichia coli |
D58A/P128A/P129A | site-directed mutagenesis, the mutant shows increased Km toward the 30S subunit by two orders of magnitude with only a marginal effect on kcat compared to the wild-type enzyme | Escherichia coli |
F130S | site-directed mutagenesis | Escherichia coli |
additional information | knockout of the rsmD gene and complementation by point mutants, except for mutant P128A/P129A/F130S, overview | Escherichia coli |
P128A/P129A | site-directed mutagenesis, the mutation decreases the enthalpic contribution to SAM binding, paralleled by an increase in the entropic contribution, | Escherichia coli |
P128A/P129A/F130S | site-directed mutagenesis, inactive mutant | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | RsmD methyltransferase kinetics, multiple turnover reaction, overview | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine966 in 16S rRNA | Escherichia coli | - |
S-adenosyl-L-homocysteine + N2-methylguanine966 in 16S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine966 in 30S rRNA | Escherichia coli | RsmD acts late in the assembly process and is able to modify a completely assembled 30S subunit | S-adenosyl-L-homocysteine + N2-methylguanine966 in 30S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | RsmD possesses superior binding properties toward the unmodified 30S subunit but is unable to bind a 30S subunit modified at G966 | Escherichia coli | ? | - |
? | |
S-adenosyl-L-methionine + guanine966 in 16S rRNA | - |
Escherichia coli | S-adenosyl-L-homocysteine + N2-methylguanine966 in 16S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine966 in 16S rRNA | RsmD recognize a single target nucleotide G966 of the 16S rRNA, substrate specificity, overview | Escherichia coli | S-adenosyl-L-homocysteine + N2-methylguanine966 in 16S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine966 in 30S rRNA | RsmD acts late in the assembly process and is able to modify a completely assembled 30S subunit | Escherichia coli | S-adenosyl-L-homocysteine + N2-methylguanine966 in 30S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine966 in 30S rRNA | RsmD-30S rRNA complex formation an structure, overview | Escherichia coli | S-adenosyl-L-homocysteine + N2-methylguanine966 in 30S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RsmD | - |
Escherichia coli |
small rRNA methyltransferase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | sinefungin is an unreactive S-adenosyl-L-methionine analogue | Escherichia coli | |
S-adenosyl-L-methionine | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
additional information | RsmD is unusual in its ability to withstand multiple amino acid substitutions of the active site. Such efficiency of RsmD may be useful to complete the modification of a 30S subunit ahead of the 30S subunit's involvement in translation | Escherichia coli |