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Literature summary for 2.1.1.175 extracted from

  • Lee, Y.; Kim, B.; Chong, Y.; Lim, Y.; Ahn, J.
    Cation dependent O-methyltransferases from rice (2008), Planta, 227, 641-647.
    View publication on PubMed
Search for more literature for 2.1.1.175

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as glutathione S-transferase fusion protein Oryza sativa

Protein Variants

Protein Variants Comment Organism
D168L mutation abolishes activity (with quercetin as substrate) Oryza sativa
D194L mutation abolishes activity (with quercetin as substrate) Oryza sativa
D209L mutation abolishes activity (with quercetin as substrate) Oryza sativa
D234L mutation abolishes activity (with quercetin as substrate) Oryza sativa
E112L mutation results in 40% loss of activity (with quercetin as substrate) Oryza sativa
E69L mutation results in 14% loss of activity (with quercetin as substrate) Oryza sativa
N195I mutation abolishes activity (with quercetin as substrate) Oryza sativa
N235I mutation abolishes activity (with quercetin as substrate) Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.036
-
 tricetin pH 7.5, 37°C Oryza sativa
0.072
-
 tricetin pH 7.5, 37°C Oryza sativa

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ the enzyme is metal-dependent, Mg2+ is the best cation for catalytic activity, Co2+ shows 41% of the activity with Mg2+ (with quercetin as substrate) Oryza sativa
Co2+ the enzyme is metal-dependent, Mg2+ is the best cation for catalytic activity, Co2+ shows 82% of the activity with Mg2+ (with quercetin as substrate) Oryza sativa
Mg2+ the enzyme is metal-dependent, Mg2+ is the best cation for catalytic activity (with quercetin as substrate) Oryza sativa
Mn2+ the enzyme is metal-dependent, Mg2+ is the best cation for catalytic activity, Mn2+ shows 69% of the activity with Mg2+ (with quercetin as substrate) Oryza sativa
Mn2+ the enzyme is metal-dependent, Mg2+ is the best cation for catalytic activity, Mn2+ shows 74% of the activity with Mg2+ (with quercetin as substrate) Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30600
-
 x * 30600, SDS-PAGE, glutathione S-transferase fusion protein Oryza sativa
31100
-
 x * 31100, SDS-PAGE, glutathione S-transferase fusion protein Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-
Oryza sativa Q9XGP7
-
-

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Oryza sativa
-
stem
-
 Oryza sativa
-
stem expressed in stems only Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + tricetin the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers. In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives. The enzyme also catalyzes the methylation of luteolin, myricetin (formation of the 3',5'-dimethyl ether of myricetin, EC 2.1.1.149) and caffeoyl-CoA. ROMT-15 exhibits similar Kcat/Km values for the four substrates. ROMT-15 can not utilize naringenin, apigenin, or kaempferol. The 2,3-double bond and the O-dihydroxyl group are both required for catalytic activity Oryza sativa 2 S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin
-
 ?
2 S-adenosyl-L-methionine + tricetin the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers. In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives. The enzyme also catalyzes the methylation of luteolin, myricetin (formation of the 3',5'-dimethyl ether of myricetin, EC 2.1.1.149) and caffeoyl-CoA. ROMT-17 prefers tricetin. ROMT-15 can not utilize naringenin, apigenin, or kaempferol. The 2,3-double bond and the O-dihydroxyl group are both required for catalytic activity Oryza sativa 2 S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin
-
 ?
S-adenosyl-L-methionine + 3'-O-methyltricetin the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers. In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives Oryza sativa S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin
-
 ?
S-adenosyl-L-methionine + tricetin the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers. In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives Oryza sativa S-adenosyl-L-homocysteine + 3'-O-methyltricetin
-
 ?

Subunits

Subunits Comment Organism
? x * 30600, SDS-PAGE, glutathione S-transferase fusion protein Oryza sativa
? x * 31100, SDS-PAGE, glutathione S-transferase fusion protein Oryza sativa

Synonyms

Synonyms Comment Organism
ROMT-15
-
 Oryza sativa
ROMT-17
-
 Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Oryza sativa

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Oryza sativa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.949
-
 tricetin pH 7.5, 37°C Oryza sativa
2.83
-
 tricetin pH 7.5, 37°C Oryza sativa