Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Hordeum vulgare |
expression in Escherichia coli | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00248 | - |
tricetin | 30°C | Hordeum vulgare | |
0.0417 | - |
tricetin | 37°C | Zea mays |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | requires no Mg2+ | Hordeum vulgare | |
additional information | requires no Mg2+ | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38700 | - |
x * 38700, calculated from sequence | Hordeum vulgare |
39600 | - |
x * 39600, calculated from sequence | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
- |
- |
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Hordeum vulgare |
- |
Zea mays |
Storage Stability | Organism |
---|---|
4°C, stable when stored in buffer containing 10% (v/v) glycerol for 3-4 weeks | Hordeum vulgare |
4°C, stable when stored in buffer containing 10% (v/v) glycerol for 34 weeks | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + tricetin | stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers (major product). The enzyme also catalyzes the 3'-O-methylation of luteolin (EC 2.1.1.42) and the methylation of 5-hydroxyferulic acid and quercetin, prefers tricetin over luteolin | Hordeum vulgare | 2 S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin | - |
? | |
2 S-adenosyl-L-methionine + tricetin | the enzyme also catalyzes the 3'-O-methylation of luteolin (EC 2.1.1.42) and the methylation of 5-hydroxyferulic acid and quercetin, prefers tricetin over luteolin | Zea mays | 2 S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin | - |
? | |
S-adenosyl-L-methionine + 3'-O-methyltricetin | the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers (major product). In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives | Hordeum vulgare | S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin | - |
? | |
S-adenosyl-L-methionine + 3'-O-methyltricetin | the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers (major product). In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives | Zea mays | S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin | - |
? | |
S-adenosyl-L-methionine + tricetin | the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers (major product). In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives | Hordeum vulgare | S-adenosyl-L-homocysteine + 3'-O-methyltricetin | - |
? | |
S-adenosyl-L-methionine + tricetin | the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers (major product). In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives | Zea mays | S-adenosyl-L-homocysteine + 3'-O-methyltricetin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38700, calculated from sequence | Hordeum vulgare |
? | x * 39600, calculated from sequence | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
HvOMT1 | - |
Hordeum vulgare |
ZmOMT1 | - |
Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Hordeum vulgare |
30 | - |
assay at | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Hordeum vulgare |
7.5 | - |
- |
Zea mays |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Zea mays | calculated from sequence | - |
5.48 |
Hordeum vulgare | calculated from sequence | - |
5.64 |