Literature summary for 2.1.1.179 extracted from

Maravic Vlahovicek, G.; Cubrilo, S.; Tkaczuk, K.L.; Bujnicki, J.M.
Modeling and experimental analyses reveal a two-domain structure and amino acids important for the activity of aminoglycoside resistance methyltransferase Sgm (2008), Biochim. Biophys. Acta, 1784, 582-590.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Micromonospora zionensis

Protein Variants

Protein Variants	Comment	Organism
D156A	mutant with drastically increased sensitivity to kanamycin	Micromonospora zionensis
D158A	mutant with drastically increased sensitivity to kanamycin	Micromonospora zionensis
D182A	mutant with drastically increased sensitivity to kanamycin	Micromonospora zionensis
E205A	mutant with drastically increased sensitivity to kanamycin	Micromonospora zionensis
E267A	mutant with drastically increased sensitivity to kanamycin	Micromonospora zionensis
G135A	mutant with drastically increased sensitivity to kanamycin	Micromonospora zionensis
K199A	mutant with drastically increased sensitivity to kanamycin	Micromonospora zionensis
K54A	mutant with drastically increased sensitivity to kanamycin	Micromonospora zionensis
additional information	analysis of sequencefunction relationships of Sgm MTase by limited proteolysis and site-directed and random mutagenesis	Micromonospora zionensis
R108A	mutant with drastically increased sensitivity to kanamycin	Micromonospora zionensis
R187S	mutant with drastically increased sensitivity to kanamycin	Micromonospora zionensis
R187S/G212S	mutant with strongly reduced activity	Micromonospora zionensis
R236A	mutant with drastically increased sensitivity to kanamycin	Micromonospora zionensis
R433A	mutant with drastically increased sensitivity to kanamycin	Micromonospora zionensis

Organism

Organism	UniProt	Comment	Textmining
Micromonospora zionensis	Q7M0R2	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Micromonospora zionensis

Reaction

Reaction	Comment	Organism	Reaction ID
S-adenosyl-L-methionine + guanine1405 in 16S rRNA = S-adenosyl-L-homocysteine + N7-methylguanine1405 in 16S rRNA	analysis of sequencefunction relationships of Sgm MTase by limited proteolysis and site-directed and random mutagenesis	Micromonospora zionensis	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + guanine1405 in 16S rRNA	-	Micromonospora zionensis	S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA	-	?

Synonyms

Synonyms	Comment	Organism
M7G1405 MTase	-	Micromonospora zionensis
methyltransferase Sgm	-	Micromonospora zionensis
Sgm MTase	-	Micromonospora zionensis

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Release 2023.1 (January 2023)