Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + adenosine1408 in 16S rRNA | Escherichia coli | the enzyme provides a panaminoglycoside-resistant nature through interference with the binding of aminoglycosides toward the A site of 16S rRNA through N-1 methylation at position A1408 | S-adenosyl-L-homocysteine + N1-methyladenosine1408 in 16S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | A8C927 | - |
- |
Purification (Comment) | Organism |
---|---|
His-tagged NpmA | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + adenine1408 in 16S rRNA | NpmA is an adenine N-1 methyltransferase specific for the A1408 position at the A site of 16S rRNA | Escherichia coli | S-adenosyl-L-homocysteine + N1-methyladenine1408 in 16S rRNA | - |
? | |
S-adenosyl-L-methionine + adenosine1408 in 16S rRNA | the enzyme provides a panaminoglycoside-resistant nature through interference with the binding of aminoglycosides toward the A site of 16S rRNA through N-1 methylation at position A1408 | Escherichia coli | S-adenosyl-L-homocysteine + N1-methyladenosine1408 in 16S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
16S rRNA m1A1408 methyltransferase | - |
Escherichia coli |
NpmA | plasmid-mediated 16S rRNA methyltransferase | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | the introduction of a recombinant plasmid carrying npmA confers on Escherichia coli consistent resistance to both 4,6-disubstituted 2-deoxystreptamines, such as amikacin and gentamicin, and 4,5-disubstituted 2-deoxystreptamines, including neomycin and ribostamycin. The enzyme provides a panaminoglycoside-resistant nature through interference with the binding of aminoglycosides toward the A site of 16S rRNA through N1-methylation at position A1408 | Escherichia coli |