Literature summary for 2.1.1.180 extracted from

Macmaster, R.; Zelinskaya, N.; Savic, M.; Rankin, C.R.; Conn, G.L.
Structural insights into the function of aminoglycoside-resistance A1408 16S rRNA methyltransferases from antibiotic-producing and human pathogenic bacteria (2010), Nucleic Acids Res., 38, 7791-7799.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Escherichia coli
expressed in Escherichia coli	Streptoalloteichus tenebrarius

Crystallization (Commentary)

Crystallization (Comment)	Organism
high-resolution crystal structures of KamB from Streptoalloteichus tenebrarius is determined at 1.69 A	Streptoalloteichus tenebrarius
high-resolution crystal structures of NpmA from Escherichia coli is determined at 1.69 A	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
D30A	mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function SAM-binding: mutant highly affected	Streptoalloteichus tenebrarius
D55A	mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function SAM-binding: mutant highly affected	Streptoalloteichus tenebrarius
E88A	mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function RNA/30S binding: mutant moderately affected	Streptoalloteichus tenebrarius
K174A	mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function RNA/30S binding: no difference to wild-type	Streptoalloteichus tenebrarius
K37A	mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function RNA/30S binding: mutant highly affected	Streptoalloteichus tenebrarius
K58A	mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function RNA/30S binding: mutant slightly affected	Streptoalloteichus tenebrarius
K63A	mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function RNA/30S binding: mutant slightly affected	Streptoalloteichus tenebrarius
K67A	mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function RNA/30S binding: mutant moderately affected	Streptoalloteichus tenebrarius
K71A	mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function RNA/30S binding: mutant moderately affected	Streptoalloteichus tenebrarius
K74A	mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function RNA/30S binding: mutant moderately affected	Streptoalloteichus tenebrarius
N138A	mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function A1408 Positioning/catalysis: mutant highly affected	Streptoalloteichus tenebrarius
R179A	mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function RNA/30S binding: mutant moderately affected	Streptoalloteichus tenebrarius
R195A	mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function RNA/30S binding: mutant moderately affected	Streptoalloteichus tenebrarius
R196A	mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function RNA/30S binding: mutant highly affected	Streptoalloteichus tenebrarius
R201A	mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function RNA/30S binding: mutant highly affected	Streptoalloteichus tenebrarius
R203A	mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function RNA/30S binding: mutant slightly affected	Streptoalloteichus tenebrarius
R60A	mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function RNA/30S binding: mutant slightly affected	Streptoalloteichus tenebrarius
R8A	mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function RNA/30S binding: mutant moderately affected	Streptoalloteichus tenebrarius
S107A	mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function RNA/30S binding: mutant slightly affected	Streptoalloteichus tenebrarius
T191A	mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function SAM-binding: mutant highly affected	Streptoalloteichus tenebrarius
W105A	mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function A1408 Positioning/catalysis: mutant highly affected	Streptoalloteichus tenebrarius
W105F	mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function A1408 Positioning/catalysis: mutant highly affected	Streptoalloteichus tenebrarius
W193A	mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function A1408 Positioning/catalysis: mutant highly affected	Streptoalloteichus tenebrarius
W193F	mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function A1408 Positioning/catalysis: mutant highly affected	Streptoalloteichus tenebrarius

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Streptoalloteichus tenebrarius	A8G927	-	-

Synonyms

Synonyms	Comment	Organism
A1408 16S rRNA methyltransferase	-	Escherichia coli
A1408 16S rRNA methyltransferase	-	Streptoalloteichus tenebrarius
KamB	-	Streptoalloteichus tenebrarius
NpmA	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	-	Escherichia coli
S-adenosyl-L-methionine	-	Streptoalloteichus tenebrarius