Cloned (Comment) | Organism |
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- |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine745 in 23S rRNA | Escherichia coli | - |
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P36999 | - |
- |
Purification (Comment) | Organism |
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- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine745 in 23S rRNA | - |
Escherichia coli | S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine745 in 23S rRNA | methylation of the N1 position of nucleotide G745 in hairpin 35 of Escherichia coli 23S ribosomal RNA. Progressive truncation of the rRNA substrate shows that structures in stem-loops 33, 34 and 35 are required for methylation by RrmA. Multiple contacts between nucleotides in these stem-loops and RrmA are confirmed in footprinting experiments. No other RrmA contact is evident elsewhere in the rRNA. The RrmA contact sites on the rRNA are inaccessible in ribosomal particles and, consistent with this, 50S subunits or 70S ribosomes are not substrates for RrmA methylation. Methylate their target nucleotides only in the free RNA | Escherichia coli | S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? |
General Information | Comment | Organism |
---|---|---|
malfunction | lack of G745 methylation results in reduced rates of protein synthesis and growth. Addition of recombinant plasmid-encoded rrmA to an rrmA-defficient strain remedies these defects | Escherichia coli |