Literature summary for 2.1.1.189 extracted from

Madsen, C.T.; Mengel-Jorgensen, J.; Kirpekar, F.; Douthwaite, S.
Identifying the methyltransferases for m(5)U747 and m(5)U1939 in 23S rRNA using MALDI mass spectrometry (2003), Nucleic Acids Res., 31, 4738-4746.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of a intein-fusion protein with an N-terminal His-tag	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P75817	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + uracil747 in 23S rRNA	it is not possible to generate a recombinant version of YbjF that retains in vitro activity, so the function of this enzyme is defined in vivo by engineering a ybjF knockout strain	Escherichia coli	S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA	-	?

Synonyms

Synonyms	Comment	Organism
RNA uridine methyltransferase B	-	Escherichia coli
RumB	-	Escherichia coli
YbjF	-	Escherichia coli

General Information

General Information	Comment	Organism
malfunction	comparison of the methylation patterns in 23S rRNAs from YbjF+ and YbjF- strains shows that the latter differs only in the lack of the 5-methyluracil747 modification	Escherichia coli

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Release 2023.1 (January 2023)