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Literature summary for 2.1.1.192 extracted from

  • Yan, F.; LaMarre, J.M.; R๖hrich, R.; Wiesner, J.; Jomaa, H.; Mankin, A.S.; Fujimori, D.G.
    RlmN and Cfr are radical SAM enzymes involved in methylation of ribosomal RNA (2010), J. Am. Chem. Soc., 132, 3953-3964.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.192

Cloned(Commentary)

Cloned (Comment) Organism
expressed as C-terminally His6-tagged enzyme, wild-type and mutant enzymes C125A, C129A and C132A Escherichia coli K-12

Protein Variants

Protein Variants Comment Organism
C125A inactive mutant enzyme Escherichia coli K-12
C129A inactive mutant enzyme Escherichia coli K-12
C132A inactive mutant enzyme Escherichia coli K-12

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the enzyme requires an intact [4Fe-S] cluster for catalysis Escherichia coli K-12

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, SDS-PAGE Escherichia coli K-12

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA Escherichia coli K-12 RlmN is an endogenous enzyme used by the cell to refine functions of the ribosome in protein synthesis S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli K-12 P36979
-
-
Escherichia coli K-12 P36979 contains the characteristic cysteine-rich CX3CX2C motif
-

Purification (Commentary)

Purification (Comment) Organism
C-terminally His6-tagged wild-type enzyme and mutant enzymes C125A, C129A and C132A Escherichia coli K-12

Reaction

Reaction Comment Organism Reaction ID
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin the enzyme catalyzes adenosine methylation by using a radical mechanism for substrate activation. The radical chemistry is enabled by the [4Fe-4S] cluster Escherichia coli K-12
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
 Escherichia coli K-12 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA
-
 ?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA RlmN is an endogenous enzyme used by the cell to refine functions of the ribosome in protein synthesis Escherichia coli K-12 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA
-
 ?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA S-adenosyl-L-methionine is both the methyl donor and the source of a 5'-deoxyadenosyl radical, which activates the substrate for methylation. The enzyme can utilize protein-free 23S rRNA as a substrate, but not the fully assembled large ribosomal subunit, suggesting that the methylations take place during the assembly of the ribosome. The key recognition elements in the 23S rRNA are helices 90-92 and the adjacent single stranded RNA that encompasses A2503 Escherichia coli K-12 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA identification of the reaction product as 2-methyladenine2503 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Escherichia coli K-12

Synonyms

Synonyms Comment Organism
RlmN
-
 Escherichia coli K-12

General Information

General Information Comment Organism
malfunction an rlmN null mutant shows slightly increased susceptibility to sparsomycin and hygromycin A Escherichia coli K-12