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Literature summary for 2.1.1.193 extracted from

  • Basturea, G.N.; Rudd, K.E.; Deutscher, M.P.
    Identification and characterization of RsmE, the founding member of a new RNA base methyltransferase family (2006), RNA, 12, 426-434.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.193

Cloned(Commentary)

Cloned (Comment) Organism
cloned as a N-terminal His-tag fusion protein, which allows easy purification, purified recombinant YggJ specifically methylates m3U1498 in vitro Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 x * 29000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + uracil1498 in 16S rRNA Escherichia coli
-
 S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AGL7 the gene is well-conserved across bacteria and plants
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + uracil1498 in 16S rRNA
-
 Escherichia coli S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
-
 ?
S-adenosyl-L-methionine + uracil1498 in 16S rRNA the enzyme is highly specific for U1498 in 16S rRNA. The purified enzyme is able to incorporate methyl groups from S-adenosyl-L-methionine into 30S particles purified from the mutant strain but not into 30S particles purified from wild type. Approximately one methyl group could be incorporated per mutant 30S subunit at high levels of enzyme Escherichia coli S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
-
 ?

Subunits

Subunits Comment Organism
? x * 29000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
DUF558 protein
-
 Escherichia coli
m3U1498 specific methyltransferase
-
 Escherichia coli
RsmE
-
 Escherichia coli
YggJ
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Escherichia coli

General Information

General Information Comment Organism
malfunction a yggJ deletion strain lacks the methyl group at U1498 as well as the specific methyltransferase activity. The deletion strain is unaffected in exponential growth in rich or minimal media at multiple temperatures, but it is defective when grown in competition with isogenic wild-type cells Escherichia coli