Activating Compound | Comment | Organism | Structure |
---|---|---|---|
spermidine | in the presence of spermidine, Mg2+ is not required for activity. Activity could be restored up to 60% of that with Mg2+ by addition of spermidine at 30 mM | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM, complete inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
uracil1498 in 16S rRNA | pH 7.0, 37°C, the Km-value for 16S rRNA is measured as a Km for the physiological substrate, 30S ribosomal subunit | Escherichia coli | |
0.0267 | - |
S-adenosyl-L-methionine | pH 7.0, 37°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | most active in the presence of 1015 mM Mg2+ at pH 79. At 30 mM Mg2+ optimal activity is reduced by 60%. In the presence of spermidine, Mg2+ is not required for activity. Activity could be restored up to 60% of that with Mg2+ by addition of spermidine at 30 mM | Escherichia coli | |
NH4+ | most active in the presence of 100 mM NH4Cl at pH 79 | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + uracil1498 in 16S rRNA | Escherichia coli | activity is dependent on a fully assembled 30 S ribosome subunit | S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AGL7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + uracil1498 in 16S rRNA | activity is dependent on a fully assembled 30 S ribosome subunit | Escherichia coli | S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA | - |
? | |
S-adenosyl-L-methionine + uracil1498 in 16S rRNA | small ribosome subunits obtained from an RsmE deletion strain can be methylated by purified RsmE, neither 70S ribosomes nor 50S subunits are active. 16S rRNA obtained from the mutant strain, synthetic 16S rRNA, and 3' minor domain RNA are all very poor or inactive as substrates. 30S particles partially depleted of proteins by treatment with high concentrations of LiCl or in vitro reconstituted intermediate particles also show little or no methyl acceptor activity. RsmE requires a highly structured ribonucleoprotein particle as a substrate for methylation | Escherichia coli | S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | forms dimers in solution | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
RsmE | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
S-adenosyl-L-methionine | pH 7.0, 37°C | Escherichia coli | |
0.0013 | - |
uracil1498 in 16S rRNA | pH 7.0, 37°C, the Km-value for 16S rRNA is measured as a Km for the physiological substrate, 30S ribosomal subunit | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Escherichia coli |