Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29168 | - |
2 * 29168, calculated from sequence | Escherichia coli |
30000 | - |
2 * 30000, SDS-PAGE in presence of 7.5% 2-mercaptoethanol | Escherichia coli |
58000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cytidine32 in tRNA | Escherichia coli | presence of 2'-O-methylated cytidine at position 32 in tRNAfMet1(CAU), tRNAfMet2(CAU), tRNASer1(UGA), and tRNATrp1(CCA). In Escherichia coli YfhQ is the only methyltransferase responsible for the formation of Cm32 in tRNA | S-adenosyl-L-homocysteine + 2'-O-methylcytidine32 in tRNA | - |
? | |
S-adenosyl-L-methionine + uridine32 in tRNA | Escherichia coli | presence of 2'-O-methylated uridine in position 32 of the anticodon loop in tRNAGln1(UUG) and tRNAGln2(CUG) | S-adenosyl-L-homocysteine + 2'-O-methyluridine32 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AE01 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no methylation of cytosine 34 in tRNALeu(CAA) | Escherichia coli | ? | - |
? | |
S-adenosyl-L-methionine + cytidine32 in tRNA | presence of 2'-O-methylated cytidine at position 32 in tRNAfMet1(CAU), tRNAfMet2(CAU), tRNASer1(UGA), and tRNATrp1(CCA). In Escherichia coli YfhQ is the only methyltransferase responsible for the formation of Cm32 in tRNA | Escherichia coli | S-adenosyl-L-homocysteine + 2'-O-methylcytidine32 in tRNA | - |
? | |
S-adenosyl-L-methionine + cytidine32 in tRNA | 2'-O-methylation of cytidine34 in tRNAGln2(CUG). Total crude tRNA extracted from the wild-type strain MC1061 was not a substrate for the purified YfhQ enzyme, while tRNA from the yfhQ K.O. strain is an excellent substrate for this enzyme | Escherichia coli | S-adenosyl-L-homocysteine + 2'-O-methylcytidine32 in tRNA | - |
? | |
S-adenosyl-L-methionine + uridine32 in tRNA | presence of 2'-O-methylated uridine in position 32 of the anticodon loop in tRNAGln1(UUG) and tRNAGln2(CUG) | Escherichia coli | S-adenosyl-L-homocysteine + 2'-O-methyluridine32 in tRNA | - |
? | |
S-adenosyl-L-methionine + uridine32 in tRNA | 2'-O-methylation of uridine34 tRNASer1(UGA). Total crude tRNA extracted from the wild-type strain MC1061 was not a substrate for the purified YfhQ enzyme, while tRNA from the yfhQ K.O. strain is an excellent substrate for this enzyme | Escherichia coli | S-adenosyl-L-homocysteine + 2'-O-methyluridine32 in tRNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 29168, calculated from sequence | Escherichia coli |
dimer | 2 * 30000, SDS-PAGE in presence of 7.5% 2-mercaptoethanol | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
TrMet(Xm32) | - |
Escherichia coli |
TrmJ | - |
Escherichia coli |
tRNA:Cm32/Um32 methyltransferase | - |
Escherichia coli |
YfhQ | - |
Escherichia coli |