Protein Variants | Comment | Organism |
---|---|---|
C271A | site-directed mutagenesis, the mutant enzyme forms an irreversible covalent crosslink between itself and the methylated cytosine in its target RNA | Homo sapiens |
additional information | the only mRNA identified by miCLIP that is differentially expressed when NSun2 is inhibited by RNAi in HEK293 cells is NSun2 itself | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the iCLIP method identifies tRNA AspGTC, ValAAC, GlyGCC, and LeuCAA as methylation substrates with methylation within the variable arm at cytosines 48, 49, and 50, no additional NSun2 target sites outside the variable arm. vtRNAs are methylation substrates for NSun2, vtRNAs are ncRNAs found as part of the vault ribonucleoprotein complex | ? | - |
? | |
S-adenosyl-L-methionine + cytosine48 in tRNAAspGTC | Homo sapiens | - |
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAAspGTC | - |
? | |
S-adenosyl-L-methionine + cytosine48 in tRNAGlyGCC | Homo sapiens | - |
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAGlyGCC | - |
? | |
S-adenosyl-L-methionine + cytosine48 in tRNALeuCAA | Homo sapiens | - |
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNALeuCAA | - |
? | |
S-adenosyl-L-methionine + cytosine49 in tRNAAspGTC | Homo sapiens | - |
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAAspGTC | - |
? | |
S-adenosyl-L-methionine + cytosine49 in tRNAGlyGCC | Homo sapiens | - |
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAGlyGCC | - |
? | |
S-adenosyl-L-methionine + cytosine49 in tRNALeuCAA | Homo sapiens | - |
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNALeuCAA | - |
? | |
S-adenosyl-L-methionine + cytosine50 in tRNAAspGTC | Homo sapiens | - |
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAAspGTC | - |
? | |
S-adenosyl-L-methionine + cytosine50 in tRNAGlyGCC | Homo sapiens | - |
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAGlyGCC | - |
? | |
S-adenosyl-L-methionine + cytosine50 in tRNALeuCAA | Homo sapiens | - |
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNALeuCAA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skin fibroblast | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the iCLIP method identifies tRNA AspGTC, ValAAC, GlyGCC, and LeuCAA as methylation substrates with methylation within the variable arm at cytosines 48, 49, and 50, no additional NSun2 target sites outside the variable arm. vtRNAs are methylation substrates for NSun2, vtRNAs are ncRNAs found as part of the vault ribonucleoprotein complex | Homo sapiens | ? | - |
? | |
additional information | development and evaluation of a customized version of the individual-nucleotide-resolution crosslinking and immunoprecipitation (iCLIP) method for detection of cytosine methylation in RNA species, site-specific methylation in tRNAs and additional messenger and noncoding RNAs (ncRNAs), overview. Identified NSun2 targets are tRNAs, mRNAs, and ncRNAs | Homo sapiens | ? | - |
? | |
S-adenosyl-L-methionine + cytosine48 in tRNAAspGTC | - |
Homo sapiens | S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAAspGTC | - |
? | |
S-adenosyl-L-methionine + cytosine48 in tRNAGlyGCC | - |
Homo sapiens | S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAGlyGCC | - |
? | |
S-adenosyl-L-methionine + cytosine48 in tRNALeuCAA | - |
Homo sapiens | S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNALeuCAA | - |
? | |
S-adenosyl-L-methionine + cytosine49 in tRNAAspGTC | - |
Homo sapiens | S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAAspGTC | - |
? | |
S-adenosyl-L-methionine + cytosine49 in tRNAGlyGCC | - |
Homo sapiens | S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAGlyGCC | - |
? | |
S-adenosyl-L-methionine + cytosine49 in tRNALeuCAA | - |
Homo sapiens | S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNALeuCAA | - |
? | |
S-adenosyl-L-methionine + cytosine50 in tRNAAspGTC | - |
Homo sapiens | S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAAspGTC | - |
? | |
S-adenosyl-L-methionine + cytosine50 in tRNAGlyGCC | - |
Homo sapiens | S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAGlyGCC | - |
? | |
S-adenosyl-L-methionine + cytosine50 in tRNALeuCAA | - |
Homo sapiens | S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNALeuCAA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NSUN2 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | sequenced cDNA libraries prepared from small RNA isolated from NSUN2+/- and NSUN2-/- human fibroblasts reveal that eight of the 43 ncRNAs are differentially regulated | additional information |
General Information | Comment | Organism |
---|---|---|
malfunction | autosomal-recessive loss of the NSUN2 gene is a causative link to intellectual disability disorders in humans. Loss of cytosine-5 methylation in vault RNAs causes aberrant processing into Argonaute-associated small RNA fragments that can function as microRNAs. Impaired processing of vault ncRNA may contribute to the etiology of NSun2-deficiency human disorders | Homo sapiens |
physiological function | no evidence for a major role of NSun2 or NSun2-mediated cytosine-5 methylation in mRNA stability | Homo sapiens |