Literature summary for 2.1.1.203 extracted from

Brzezicha, B.; Schmidt, M.; Makalowska, I.; Jarmolowski, A.; Pienkowska, J.; Szweykowska-Kulinska, Z.
Identification of human tRNA:m5C methyltransferase catalysing intron-dependent m5C formation in the first position of the anticodon of the pre-tRNA Leu(CAA) (2006), Nucleic Acids Res., 34, 6034-6043.

Search for more literature for 2.1.1.203

Application

Application	Comment	Organism
medicine	this methyltransferase is a novel downstream Myctarget which mediates Myc-induced cell proliferation and growth. Therefore, the characterization of hTrm4 substrate specificity can be essential since this enzyme is a potential target for cancer therapies	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the human Trm4 (hTrm4) cDNA in yeast partially complements the lack of the endogenous Trm4p enzyme	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
nucleolus	-	Homo sapiens	5730	-
nucleoplasm	-	Homo sapiens	5654	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
86471	-	x * 86471, calculated from sequence	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + cytosine34 in tRNA	Homo sapiens	specific modification of cytosine34 in the intron-containing yeast pre-tRNALeu(CAA)	S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q08J23	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
S-adenosyl-L-methionine + cytosine34 in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor	the formation of 5-methylcytosine34 is a strictly intron-dependent process	Homo sapiens	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + cytosine34 in tRNA	specific modification of cytosine34 in the intron-containing yeast pre-tRNALeu(CAA)	Homo sapiens	S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor	-	?
S-adenosyl-L-methionine + cytosine34 in tRNA precursor	specific modification of cytosine34 in the intron-containing yeast pre-tRNALeu(CAA). The hTrm4 MTase has a much narrower specificity against the yeast substrates than its yeast orthologue. The human enzyme is not able to form 5-methylcytosine34 at positions 48 and 49 of human and yeast tRNA precursors. Intron in the human pre-tRNALeu(CAA) is indispensable for the C5-methylation of cytosine in the first position of the anticodon. The modification of C34 depends on the nucleotide sequence surrounding the position to be modified and on the structure of intron-containing prolongated anticodon stem	Homo sapiens	S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor	-	?

Subunits

Subunits	Comment	Organism
?	x * 86471, calculated from sequence	Homo sapiens

Synonyms

Synonyms	Comment	Organism
hTrm4	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Homo sapiens

General Information

General Information	Comment	Organism
physiological function	specific modification of cytosine34 in the intron-containing yeast pre-tRNALeu(CAA)	Homo sapiens

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Release 2023.1 (January 2023)